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利用扩增片段长度多态性(AFLP)和反转录转座子插入多态性(IRAP)分子标记阐明伊朗油橄榄种质的遗传多样性和群体结构。

Elucidate genetic diversity and population structure of Olea europaea L. germplasm in Iran using AFLP and IRAP molecular markers.

作者信息

Khaleghi Esmaeil, Sorkheh Karim, Chaleshtori Maryam Hosseni, Ercisli Sezai

机构信息

Department of Horticultural Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box 61355/144, Ahvaz, Iran.

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box 61355/144, Ahvaz, Iran.

出版信息

3 Biotech. 2017 May;7(1):71. doi: 10.1007/s13205-017-0669-x. Epub 2017 Apr 27.

Abstract

Currently, study of the inter and the intra-population genetic disparity was done by use of the 200 Olea europaea L. which is found growing naturally in the nation of Iran, and this study was carried out by AFLP and IRAP markers. The fingerprints that were similar to the AFLP and the IRAP markers were evidence of high concentrations of heterozygosity and this shows that O. europaea L. is primarily the out crossing species. The average percentage of polymorphism is as shown below: 87.15 and 87.38% of the information used in regard to the AFLP and the IRAP, respectively. The gene disparity numerals on the population researched were 1.087 for HT and 0.871 for HS in regard to AFLP. For the IRAP it was 1.084 for HT and 0.860 for HS. The general values for genetic variations that are found in the O. europaea L. germplasm in the nation of Iran were then assessed through putting together the AFLP and the IRAP information so as to cover a larger genome. Arguing from the AFLP and the IRAP studies, it can be concluded that there are more levels of genetic variation at inter and the intra-population level for the O. europaea.

摘要

目前,利用在伊朗自然生长的200株油橄榄开展了种群间和种群内遗传差异研究,该研究通过AFLP和IRAP标记进行。与AFLP和IRAP标记相似的指纹图谱是杂合度高的证据,这表明油橄榄主要是异交物种。多态性的平均百分比如下:分别用于AFLP和IRAP的信息中,多态性分别为87.15%和87.38%。就AFLP而言,所研究种群的基因差异数值,HT为1.087,HS为0.871。就IRAP而言,HT为1.084,HS为0.860。然后通过整合AFLP和IRAP信息以覆盖更大的基因组,评估了伊朗油橄榄种质中发现的遗传变异的总体值。从AFLP和IRAP研究来看,可以得出结论,油橄榄在种群间和种群内水平上存在更多的遗传变异水平。

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