la Rosa R, Angiolillo A, Guerrero C, Pellegrini M, Rallo L, Besnard G, Bervillé A, Martin A, Baldoni L
Dpto. Agronomia - Universidad de Córdoba, Av.da Menendez Pidal, S/N, Spain.
Theor Appl Genet. 2003 May;106(7):1273-82. doi: 10.1007/s00122-002-1189-5. Epub 2003 Feb 11.
The first linkage map of the olive (Olea europaea L.) genome has been constructed using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLP) as dominant markers and a few restriction fragment length polymorphisms (RFLP) and simple-sequence repeats (SSR) as codominant markers. Ninety-five individuals of a cross progeny derived from two highly heterozygous olive cultivars, Leccino and Dolce Agogia, were used by applying the pseudo test-cross strategy. From 61 RAPD primers 279 markers were obtained - 158 were scored for Leccino and 121 for Dolce Agogia. Twenty-one AFLP primer combinations gave 304 useful markers - 160 heterozygous in Leccino and 144 heterozygous in Dolce Agogia. In the Leccino map 249 markers (110 RAPD, 127 AFLP, 8 RFLP and 3 SSR) were linked. This resulted in 22 major linkage groups and 17 minor groups with fewer than four markers. In the Dolce Agogia map, 236 markers (93 RAPD, 133 AFLP, 6 RFLP and 4 SSR) were linked; 27 major linkage groups and three minor groups were obtained. Codominant RFLPs and SSRs, as well as few RAPDs in heteroduplex configuration, were used to establish homologies between linkage groups of both parents. The total distance covered was 2,765 cM and 2,445 cM in the Leccino and Dolce Agogia maps, respectively. The mean map distance between adjacent markers was 13.2 cM in Leccino and 11.9 cM in Dolce Agogia, respectively. Both AFLP and RAPD markers were homogeneously distributed in all of the linkage groups reported. The stearoyl-ACP desaturase gene was mapped on linkage group 4 of cv. Leccino.
利用随机扩增多态性DNA（RAPD）和扩增片段长度多态性（AFLP）作为显性标记，以及少数限制性片段长度多态性（RFLP）和简单序列重复（SSR）作为共显性标记，构建了橄榄（油橄榄）基因组的首张连锁图谱。通过应用拟测交策略，使用了由两个高度杂合的橄榄品种莱基诺（Leccino）和多奇阿戈亚（Dolce Agogia）杂交产生的95个后代个体。从61个RAPD引物中获得了279个标记——莱基诺有158个可计分标记，多奇阿戈亚有121个。21个AFLP引物组合产生了304个有用标记——莱基诺中有160个杂合标记，多奇阿戈亚中有144个杂合标记。在莱基诺图谱中，249个标记（110个RAPD、127个AFLP、8个RFLP和3个SSR）被连锁在一起。这产生了22个主要连锁群和17个标记数少于4个的小连锁群。在多奇阿戈亚图谱中，236个标记（93个RAPD、133个AFLP、6个RFLP和4个SSR）被连锁；得到了27个主要连锁群和3个小连锁群。共显性的RFLP和SSR，以及少数处于异源双链构型的RAPD，被用于建立双亲连锁群之间的同源性。莱基诺和多奇阿戈亚图谱覆盖的总距离分别为2765厘摩和2445厘摩。莱基诺图谱中相邻标记之间的平均图谱距离为13.2厘摩，多奇阿戈亚图谱中为11.9厘摩。AFLP和RAPD标记在所有报道的连锁群中均呈均匀分布。硬脂酰-ACP去饱和酶基因被定位在莱基诺品种的第4连锁群上。
