Chang R J, Smilowitz H
Department of Pharmacology, University of Connecticut Health Center, Farmington 06032.
Life Sci. 1988;43(13):1055-61. doi: 10.1016/0024-3205(88)90200-7.
Our newly isolated monoclonal antibody (#78) specifically interacts with the 170Kd 1,4 dihydropyridine binding component of the skeletal muscle calcium channel. Dihydropyridine receptor (DHPR) from rabbit skeletal muscle and canine cardiac membranes were purified by monoclonal antibody #78 affinity chromatography. We show that DHPR from canine cardiac membranes like DHPR from rabbit skeletal membranes contain a approximately 170Kd polypeptide to which antibody #78 immunoblots under both reducing and non-reducing conditions.
我们新分离出的单克隆抗体（#78）可特异性地与骨骼肌钙通道的170Kd 1,4 - 二氢吡啶结合成分相互作用。兔骨骼肌和犬心肌膜中的二氢吡啶受体（DHPR）通过单克隆抗体#78亲和层析法进行纯化。我们发现，犬心肌膜中的DHPR与兔骨骼肌膜中的DHPR一样，在还原和非还原条件下，均含有一条约170Kd的多肽，抗体#78可对其进行免疫印迹分析。
