Liu Cong, Liu Jing, Hao Yuhui, Gu Ying, Yang Zhangyou, Li Hong, Li Rong
a Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine , College of Preventive Medicine, Third Military Medical University , Chongqing , China.
Int J Radiat Biol. 2017 Aug;93(8):793-802. doi: 10.1080/09553002.2017.1321808. Epub 2017 May 19.
6,7,3',4'-tetrahydroxyisoflavone (T3) is a novel chemically synthesized compound reported in our previous study. This study was designed to explore the radioprotective effect of T3, and if so, its potential mechanisms.
KunMing mice were exposed to various doses of γ irradiation (60 Co) after being treated with dimethyl sulfoxide (DMSO) or T3. Briefly, survival rate, dose reducing factor (DRF), body weight change (%), spleen index (SI) and thymus index (TI) of irradiated mice with or without different doses of T3 treatment were evaluated routinely. The hematopoietic function of bone marrow was emphatically investigated. In vitro experiments were performed to observe the protective effect of T3 on irradiated human lymphocyte AHH-1 cells by cell viability or flow cytometry (FCM) assays.
A single dose of subcutaneous administration of T3 significantly improved the survival rate, and enhanced the restoration of hematopoietic function in irradiated mice. T3 also decreased the apoptosis of irradiated AHH-1 cells in vitro.
T3 protected mice against lethal γ irradiation-induced injury probably through the restoration of hematopoietic function. This implied that T3 could be further developed as a radioprotector.
6,7,3',4'-四羟基异黄酮（T3）是我们先前研究中报道的一种新型化学合成化合物。本研究旨在探讨T3的辐射防护作用，若有，其潜在机制。
昆明小鼠在经二甲基亚砜（DMSO）或T3处理后，接受不同剂量的γ射线（60Co）照射。简要地说，常规评估接受或未接受不同剂量T3处理的受照射小鼠的存活率、剂量减低因子（DRF）、体重变化（%）、脾脏指数（SI）和胸腺指数（TI）。重点研究骨髓的造血功能。进行体外实验，通过细胞活力或流式细胞术（FCM）检测观察T3对受照射人淋巴细胞AHH-1细胞的保护作用。
单次皮下注射T3可显著提高受照射小鼠的存活率，并增强其造血功能的恢复。T3还可降低体外受照射AHH-1细胞的凋亡率。
T3可能通过恢复造血功能来保护小鼠免受致死性γ射线照射所致损伤。这意味着T3可进一步开发成为一种辐射防护剂。
