Iwata A, Watanabe Y, Sakata T, Sokawa Y, Kawade Y
Institute for Virus Research, Kyoto University.
Microbiol Immunol. 1988;32(6):597-605. doi: 10.1111/j.1348-0421.1988.tb01421.x.
A mouse leukemic cell line L1210 Sg with a low sensitivity to interferon-gamma (IFN-gamma) was described. On the nature of the antiviral action and binding of IFN, L1210 Sg cells were compared with L1210 m cell line which is sensitive to IFN-gamma. For a half reduction of the vesicular stomatitis virus-RNA synthesis, L1210 Sg cells required 500-fold more IFN-gamma than L1210 m cells did. However, both cell lines were induced to the antiviral state to the same extent with IFN-alpha or -beta. L1210 Sg and L1210 m cells were sensitive to the anti-proliferative action of IFN-alpha and -beta, but insensitive to IFN-gamma. (2'-5')Oligoadenylate synthetase was induced in these cell lines by IFN-beta, but not by IFN-gamma, which suggests that the induction of this synthetase may not be responsible for the antiviral action of IFN-gamma. No substantial difference between L1210 Sg and L1210 m cells was found in IFN receptors for IFN-gamma and IFN-beta either in number per cell or in their affinity to corresponding IFN type. However, differences were noted in time course profiles of cell-associated IFN-gamma at 37 C: in L1210 m cells, a rise-and-decay profile of IFN-gamma bound to cells was observed at 37 C, but in L1210 Sg cells, rise and slight decay was observed. On the other hand, a similar rise-and-decay curve of IFN-beta bound to these cells was observed. These results indicated that the low sensitivity of L1210 Sg cells to IFN-gamma may be due to this slight decay of receptor-bound IFN-gamma.
已描述了一种对γ干扰素（IFN-γ）敏感性较低的小鼠白血病细胞系L1210 Sg。在抗病毒作用和IFN结合的性质方面，将L1210 Sg细胞与对IFN-γ敏感的L1210 m细胞系进行了比较。为使水疱性口炎病毒-RNA合成减少一半，L1210 Sg细胞所需的IFN-γ比L1210 m细胞多500倍。然而，两种细胞系用α或β干扰素诱导到抗病毒状态的程度相同。L1210 Sg和L1210 m细胞对α和β干扰素的抗增殖作用敏感，但对γ干扰素不敏感。(2'-5')寡腺苷酸合成酶在这些细胞系中由β干扰素诱导，但不由γ干扰素诱导，这表明该合成酶的诱导可能与γ干扰素的抗病毒作用无关。在L1210 Sg和L1210 m细胞之间，无论是每细胞的γ干扰素和β干扰素受体数量，还是它们与相应IFN类型的亲和力，均未发现实质性差异。然而，在37℃时细胞相关γ干扰素的时间进程曲线存在差异：在L1210 m细胞中，37℃时观察到与细胞结合的γ干扰素呈上升-下降曲线，但在L1210 Sg细胞中，观察到上升和轻微下降。另一方面，观察到与这些细胞结合的β干扰素呈现类似的上升-下降曲线。这些结果表明，L1210 Sg细胞对γ干扰素的低敏感性可能是由于受体结合的γ干扰素的这种轻微下降所致。
