Ekaney Michael L, Grable Martin A, Powers William F, McKillop Iain H, Evans Susan L
From the Department of Surgery (M.L.E., W.F.P., I.H.M., S.L.E.), FH "Sammy" Ross Trauma Center, Carolinas Medical Center; and Community Blood Center of the Carolinas (M.A.G.), Charlotte, NC.
J Trauma Acute Care Surg. 2017 Aug;83(2):271-277. doi: 10.1097/TA.0000000000001547.
Donated platelets are stored at 22°C and discarded within 5 days because of diminished function and risk of bacterial contamination. Decline of platelet function has been attributed to decreased mitochondrial function and increased oxidative stress. Resveratrol (Res) and cytochrome c (Cyt c), in combination with hypothermic storage, may extend platelet viability.
Platelets from 20 donors were pooled into four independent sets and stored at 22°C or 4°C in the absence or presence of Res (50 μM) or Cyt c (100 μM) for up to 10 days. Sequential measurement of platelet counts, coagulation function (thromboelastography), oxygen consumption, lipid peroxidation, glucose-lactate levels, pH, TCO2, and soluble platelet activation markers (CD62P/PF-4) was performed.
Platelet function diminished rapidly over time at 22°C versus 4°C (adenosine diphosphate, day 10 [0.6 ± 0.5] vs. [7.8 ± 3.5], arachidonic acid: day 10 [0.5 ± 0.5] vs. [30.1 ± 27.72]). At 4°C, storage treatment with Res or Cyt c limited deterioration in platelet function up to day 10, an effect not observed at 22°C (day 10, 4°C, Con [7.8 ± 3.5] vs. Res [37.3 ± 24.19] vs. Cyt c [45.83 ± 43.06]). Mechanistic analysis revealed oxygen consumption increased in response to Cyt c at 22°C, whereas neither Cyt c or Res affected oxygen consumption at 4°C. Lipid peroxidation was only reduced at 22°C (day 7 and day 10), but remained unchanged at 4°C, or when Res or Cyt c was added. Cytosolic ROS was significantly reduced by pretreatment with Res at 4°C. Total platelet count and soluble activation markers were unchanged during storage and not affected by Res, Cyt c, or temperature. Glucose concentration, pH and TCO2 decreased while lactate levels increased during storage at 22°C but not 4°C.
Platelet function is preserved by cold storage for up to 10 days. This function is enhanced by treatment with Res or Cyt c, which supports mitochondrial activity, thus potentially extending platelet shelf life.
捐献的血小板在22°C下储存，并在5天内丢弃，因为其功能会下降且存在细菌污染风险。血小板功能的下降归因于线粒体功能降低和氧化应激增加。白藜芦醇（Res）和细胞色素c（Cyt c）与低温储存相结合，可能会延长血小板的存活期。
将来自20名捐献者的血小板汇集到四个独立组中，在不存在或存在Res（50μM）或Cyt c（100μM）的情况下于22°C或4°C储存长达10天。对血小板计数、凝血功能（血栓弹力图）、耗氧量、脂质过氧化、葡萄糖-乳酸水平、pH、总二氧化碳（TCO2）和可溶性血小板活化标志物（CD62P/PF-4）进行连续测量。
与4°C相比，血小板功能在22°C下随时间迅速下降（二磷酸腺苷，第10天[0.6±0.5]对[7.8±3.5]，花生四烯酸：第10天[0.5±0.5]对[30.1±27.72]）。在4°C下，用Res或Cyt c进行储存处理可在第10天之前限制血小板功能的恶化，在22°C下未观察到这种效果（第10天，4°C，对照组[7.8±3.5]对Res组[37.3±24.19]对Cyt c组[45.83±43.06]）。机制分析显示，在22°C下，细胞色素c会使耗氧量增加，而在4°C下，细胞色素c和白藜芦醇均不影响耗氧量。脂质过氧化仅在22°C下（第7天和第10天）有所降低，但在4°C下或添加Res或Cyt c时保持不变。在4°C下，用Res预处理可显著降低胞质活性氧（ROS）。储存期间血小板总数和可溶性活化标志物未发生变化，且不受Res、Cyt c或温度的影响。在22°C而非4°C储存期间，葡萄糖浓度、pH和TCO2降低，而乳酸水平升高。
血小板功能通过冷藏可保存长达10天。Res或Cyt c处理可增强此功能，这两种物质可支持线粒体活性，从而有可能延长血小板的保质期。
