Research and Development, Australian Red Cross Lifeblood (formerly the Australian Red Cross Blood Service), Alexandria, New South Wales, Australia.
Faculty of Science, School of Life Sciences and Proteomics Core Facility, University of Technology Sydney, Sydney, New South Wales, Australia.
Transfusion. 2020 Dec;60(12):2969-2981. doi: 10.1111/trf.16127. Epub 2020 Oct 21.
Cryopreservation of platelets with dimethylsulfoxide (DMSO) at -80°C increases their shelf life from days to years. Once thawed, platelets are stored at room temperature (RT), and the shelf life is limited to 4-6 hours. However, refrigeration (cold storage) may facilitate a prolongation of the shelf life of thawed platelets.
ABO-matched buffy coat-derived platelets (30% plasma/70% SSP+) were cryopreserved at -80°C in 5%-6% DMSO. Paired cryopreserved platelet components were thawed, resuspended in 30% plasma/70% SSP+, and then stored at either 20°C-24°C with agitation (RT) or at 2°C-6°C (cold). In vitro platelet quality was assessed over 10 days of postthaw storage.
During postthaw storage, the platelet concentration of RT-stored components decreased significantly more than components in cold storage (Day 10 RT 58 ± 10 × 10 /unit vs Day 10 cold 142 ± 16 × 10 /unit; P < .0001). Cold storage reduced the metabolic rate of thawed platelets. During storage, the surface glycoprotein ([GP] Ibα, GPVI, GPIIb, GPIIIa) and activation marker (P-selectin and phosphatidylserine) profile of cold platelets was closer to freshly thawed platelets (Day 0) than those stored at RT. Thromboelastography (reaction time) demonstrated that the procoagulant nature of cryopreserved platelets was preserved during 10 days of cold storage, while RT-stored thawed platelets displayed a gradual prolongation of the time taken to initiate clot formation.
Cold storage of thawed platelets preserves the platelet phenotype and function for up to 10 days, compared to thawed platelets stored at RT. Thus, cold storage of thawed platelets may represent a simple approach to extend the postthaw shelf life.
用二甲基亚砜（DMSO）将血小板在-80°C 下冷冻保存，可将其保存期限从数天延长至数年。一旦解冻，血小板就存放在室温（RT）下，保存期限限于 4-6 小时。然而，冷藏（低温储存）可能有助于延长解冻后的血小板保存期限。
将 ABO 匹配的富血小板血浆（30%血浆/70% SSP+）在-80°C 下用 5%-6% DMSO 冷冻保存。将配对的冷冻血小板成分解冻，重悬于 30%血浆/70% SSP+中，然后分别在 20°C-24°C 搅拌（RT）或 2°C-6°C（冷）下储存。在解冻后储存的 10 天内，评估血小板的体外质量。
在解冻后储存期间，RT 储存的血小板浓度比冷储存的血小板浓度显著下降更多（第 10 天 RT 为 58±10×10 /单位，而第 10 天冷储为 142±16×10 /单位；P <.0001）。低温储存降低了解冻血小板的代谢率。在储存期间，冷储血小板的表面糖蛋白（[GP] Ibα、GPVI、GPIIb、GPIIIa）和激活标志物（P-选择素和磷脂酰丝氨酸）谱比 RT 储存的血小板更接近新鲜解冻的血小板（第 0 天）。血栓弹性描记术（反应时间）表明，冷冻保存的血小板的促凝性质在 10 天的冷储存期间得以保留，而 RT 储存的解冻血小板显示出启动血栓形成所需时间的逐渐延长。
与 RT 储存的解冻血小板相比，冷储可将解冻血小板的血小板表型和功能保存长达 10 天。因此，冷储解冻血小板可能是延长解冻后保存期限的一种简单方法。
