大鼠脑毛细血管中特异性心房利钠肽结合位点

Specific atrial natriuretic peptide binding sites in rat cerebral capillaries.

作者信息

Niwa M, Ibaragi M, Tsutsumi K, Kurihara M, Himeno A, Mori K, Ozaki M

机构信息

Department of Pharmacology, Nagasaki University School of Medicine, Japan.

出版信息

Neurosci Lett. 1988 Aug 15;91(1):89-94. doi: 10.1016/0304-3940(88)90254-6.

DOI:10.1016/0304-3940(88)90254-6

PMID:2845310

Abstract

We examined the specific rat 125I-alpha-rat atrial natriuretic peptide(1-28)[ANF-(99-126)] (125I-rANP) binding sites in the cerebral capillaries from the cerebral cortex of male adult Wistar rats. The binding of 125I-rANP at 37 degrees C was saturable and of high affinity with a Kd of approximately 100 pM and Bmax of 152 fmol/mg protein. Divalent cations, Mn2+ (2.2 mM) and Ca2+ (1.8 mM) potently inhibited the binding. The rank order for inhibition of the binding was rANP, alpha-human ANP and ANF-(101-126) greater than ANF-(103-126) and ANF-(103-125)"ANF-(103-123). These data on specific binding sites of ANP in cerebral capillaries suggest a possible role for ANP in the blood-brain permeability of water and electrolytes.

摘要

我们检测了成年雄性Wistar大鼠大脑皮质脑毛细血管中特异性大鼠125I-α-大鼠心钠素（1-28）[心钠素-（99-126）]（125I-rANP）结合位点。37℃时125I-rANP的结合具有饱和性且亲和力高，解离常数（Kd）约为100 pM，最大结合容量（Bmax）为152 fmol/mg蛋白质。二价阳离子Mn2+（2.2 mM）和Ca2+（1.8 mM）强烈抑制这种结合。抑制结合的活性顺序为：rANP、α-人ANP和心钠素-（101-126）大于心钠素-（103-126）和心钠素-（103-125）＞心钠素-（103-123）。这些关于脑毛细血管中心钠素特异性结合位点的数据提示心钠素在水和电解质的血脑通透性方面可能发挥作用。