Hsieh Sung-Yu, Wang Chun-Chi, Kou Hwang-Shang, Wu Shou-Mei
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan.
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan; Department of Chemistry, College of Sciences, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan; Food and Drug Administration, Ministry of Health and Welfare, Taiwan.
J Pharm Biomed Anal. 2017 Jul 15;141:222-228. doi: 10.1016/j.jpba.2017.04.013. Epub 2017 Apr 15.
The beta-adrenergic agonists (β-agonists) working as repartitioning agents that make the carcass leaner and enhance the feeding efficiency in animals have been banned in the European Union, China and Taiwan. Here, traditional anionic surfactants, such as sodium dodecyl sulfate (SDS) were replaced with sodium di-(2-ethylhexyl)-sulfosuccinate (AOT) in field-amplified sample injection and sweeping-micellar electrokinetic chromatography (FASI-sweeping MEKC) for simultaneous analysis of eight β-agonists in animal feeds. The AOT vesicles provided a better resolution of β-agonists than micelles of SDS. The detection limits of the eight β-agonists were above 5ng/mL by using this stacking capillary electrophoresis (CE) method. In comparison of traditional MEKC method (sample injection, 1psi for 5s), the stacking strategy provided 400-2000 fold sensitivity enhancement. After method validation, this method was successfully applied for analyzing four animal feeds, and none β-agonist was detected. This strategy possessing good resolution of eight β-agonists was suitable for serving as a tool for routine analysis of animal feeds.
作为使动物胴体更瘦并提高其饲料效率的重分配剂的β-肾上腺素能激动剂（β-激动剂）已在欧盟、中国和台湾被禁用。在此，在场放大进样和扫集胶束电动色谱法（FASI-扫集MEKC）中，用二（2-乙基己基）磺基琥珀酸钠（AOT）取代了传统的阴离子表面活性剂，如十二烷基硫酸钠（SDS），用于同时分析动物饲料中的八种β-激动剂。与SDS胶束相比，AOT囊泡对β-激动剂具有更好的分离效果。使用这种堆积毛细管电泳（CE）方法，八种β-激动剂的检测限均高于5ng/mL。与传统的MEKC方法（进样，1psi持续5秒）相比，堆积策略使灵敏度提高了400 - 2000倍。经过方法验证后，该方法成功应用于分析四种动物饲料，未检测到β-激动剂。这种对八种β-激动剂具有良好分离效果的策略适合作为动物饲料常规分析的工具。
