Hashemi Mohammad, Amininia Shadi, Ebrahimi Mahboubeh, Simforoosh Nasser, Basiri Abbas, Ziaee Seyed Amir Mohsen, Narouie Behzad, Sotoudeh Mehdi, Mollakouchekian Mohammad Javad, Rezghi Maleki Esmaeil, Hanafi-Bojd Hamideh, Rezaei Maryam, Bahari Gholamreza, Taheri Mohsen, Ghavami Saeid
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43181, Iran.
Oncol Lett. 2017 Apr;13(4):2483-2489. doi: 10.3892/ol.2017.5739. Epub 2017 Feb 14.
Tumor protein 53 (), a tumor suppressor gene, is a vital cellular cancer suppressor in multicellular organisms. Murine double minute-2 (MDM2) is an oncoprotein that inhibits TP53 activity. A number of studies have examined the association of and polymorphisms with the risk of common forms of cancer, but the findings remain inconclusive. The present study aimed to evaluate the impact of the 40-bp insertion/deletion (I/D) polymorphism (rs3730485) in the promoter region and the 16-bp I/D polymorphism (rs17878362) in on the susceptibility of prostate cancer (PCa) in a sample of the Iranian population. This case-control study included 103 patients with pathologically confirmed PCa and 142 patients with benign prostatic hyperplasia. The 40-bp I/D and 16-bp I/D polymorphism was determined using polymerase chain reaction analysis. The results demonstrated that the 40-bp I/D polymorphism increased the risk of PCa in a co-dominant inheritance model [odds ratio (OR)=1.88; 95% confidence interval (CI)=1.11-3.19; P=0.023, D/D vs. I/I], while this variant marginally increased the risk of PCa in a dominant model (OR=1.69; 95% CI=1.00-2.83; P=0.051, I/D+D/D vs. I/I). No significant association was observed between the 16-bp I/D polymorphism and PCa. In conclusion, the present study demonstrated that the 40-bp I/D polymorphism in the promoter increased the risk of PCa in an Iranian population. Further investigations with diverse ethnicities and larger sample sizes are required to verify these results.
肿瘤蛋白53（TP53）是一种肿瘤抑制基因，是多细胞生物中重要的细胞癌症抑制因子。小鼠双微体2（MDM2）是一种抑制TP53活性的癌蛋白。许多研究探讨了TP53和MDM2基因多态性与常见癌症风险的关联，但结果仍无定论。本研究旨在评估TP53启动子区域40bp插入/缺失（I/D）多态性（rs3730485）和MDM2基因16bp I/D多态性（rs17878362）对伊朗人群样本中前列腺癌（PCa）易感性的影响。这项病例对照研究纳入了103例经病理确诊的PCa患者和142例良性前列腺增生患者。采用聚合酶链反应分析确定TP53基因40bp I/D和MDM2基因16bp I/D多态性。结果表明，在共显性遗传模型中，TP53基因40bp I/D多态性增加了PCa风险[比值比（OR）=1.88；95%置信区间（CI）=1.11 - 3.19；P = 0.023，D/D vs. I/I]，而在显性模型中该变异略微增加了PCa风险（OR = 1.69；95% CI = 1.00 - 2.83；P = 0.051，I/D + D/D vs. I/I）。未观察到MDM2基因16bp I/D多态性与PCa之间存在显著关联。总之，本研究表明TP53启动子区域的40bp I/D多态性增加了伊朗人群患PCa的风险。需要进一步开展不同种族和更大样本量的研究来验证这些结果。
