Chen Xiuqing, Zhang Xianming, Zhang Jie, Gao Yang, Yang Zhaohui, Li Shanshan, Dai Haiwen
Department of Emergency, Zhejiang Hospital, 12 Lingyin Road, Hangzhou, 310013, China.
Department of Respiratory Medicine, First Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China.
BMC Complement Altern Med. 2017 Apr 28;17(1):234. doi: 10.1186/s12906-017-1747-7.
Acute lung injury (ALI) is an inflammatory disorder. Semen Cassiae has potent anti-inflammatory activities. The aim of our study was to investigate whether Semen Cassiae plays a protective effect on lipopolysaccharide (LPS)-induced ALI and, if so, to elucidate its potential mechanism.
Male Sprague-Dawley rat lungs were injured by intratracheal instillation of LPS. Rats were treated with Semen Cassiae or vehicle 3 h after LPS challenge. Samples were harvested 24 h post-LPS administration. We also investigated the effects of Semen Cassiae on LPS stimulation in RAW 264.7 cells.
LPS administration markedly induced pulmonary edema and polymorphonuclear neutrophil influxes. These changes were significantly attenuated in Semen Cassiae treated group. Moreover, Semen Cassiae markedly reduced pulmonary interleukin (IL)-6, tumor necrosis factor (TNF)-α, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. The pulmonary soluble epoxide hydrolase (sEH) activity and the DNA binding activity of Nuclear factor (NF)-κB were significantly inhibited in Semen Cassiae treated group. Furthermore, Semen Cassiae treatment significantly increased epoxyeicosatrienoic acids (EETs), and heme oxygenase-1 (HO-1) activity. Our in vitro study demonstrates that Semen Cassiae treatment may inhibit LPS induced IκBα phosphorylation and NF-κB p65 nucleus translocation.
Semen Cassiae protects LPS-induced ALI in rats. Semen Cassiae can be developed as a novel treatment for ALI.
急性肺损伤（ALI）是一种炎症性疾病。决明子具有强大的抗炎活性。我们研究的目的是调查决明子是否对脂多糖（LPS）诱导的ALI具有保护作用，如果是，则阐明其潜在机制。
通过气管内滴注LPS损伤雄性Sprague-Dawley大鼠的肺。在LPS攻击后3小时用决明子或赋形剂治疗大鼠。在LPS给药后24小时收集样本。我们还研究了决明子对RAW 264.7细胞中LPS刺激的影响。
给予LPS显著诱导肺水肿和多形核中性粒细胞浸润。这些变化在决明子治疗组中显著减轻。此外，决明子显著降低了肺白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α和8-羟基-2'-脱氧鸟苷（8-OHdG）水平。决明子治疗组的肺可溶性环氧化物水解酶（sEH）活性和核因子（NF）-κB的DNA结合活性显著受到抑制。此外，决明子治疗显著增加了环氧二十碳三烯酸（EETs）和血红素加氧酶-1（HO-1）活性。我们的体外研究表明，决明子治疗可能抑制LPS诱导的IκBα磷酸化和NF-κB p65核转位。
决明子可保护大鼠免受LPS诱导的ALI。决明子可开发成为一种治疗ALI的新方法。
