Pradhan Madhusmita, Sahoo Ranjan Kumar, Pradhan Chinmay, Tuteja Narendra, Mohanty Santanu
Department of Soil Science and Agricultural Chemistry, College of Agriculture, OUAT, Bhubaneswar, Odisha, India.
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna AsafAli Marg, New Delhi, India.
Protoplasma. 2017 Nov;254(6):2225-2236. doi: 10.1007/s00709-017-1112-1. Epub 2017 Apr 28.
The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with pH ≤ 5.50 were collected from five districts of Odisha viz., Balasore, Cuttack, Khordha, Keonjhar, and Mayurbhanj. One bacterial isolate from each district were selected and analyzed for their P solubilization efficiency in National Botanical Research Institute Phosphate broths with Ca, Al, and Fe-complexed phosphates. CTC12 and KHD08 transformed more amount of soluble P from Ca-P (CTC12 393.30 mg/L; KHD08 465.25 mg/L), Al-P (CTC12 40.00 mg/L; KHD08 34.50 mg/L), Fe(III)-P (CTC12 175.50 mg/L; KHD08 168.75 mg/L), and Fe(II)-P (CTC12 47.40 mg/L; KHD08 42.00 mg/L) after 8 days of incubation. The bioconversion of P by all the five strains in the broth medium followed the order Ca-P > Fe(III)-P > Fe(II)-P > Al-P. The identified five strains were Bacillus cereus BLS18 (KT582541), Bacillus amyloliquefaciens CTC12 (KT633845), Burkholderia cepacia KHD08 (KT717633), B. cepacia KJR03 (KT717634), and B. cepacia K1 (KM030037) and further studied for biofertilization effects on peanut. CTC12 and KHD08 enhanced the soil available P around 65 and 58% and reduced the amount of each Al about 79 and 81%, respectively, over the uninoculated control pots in the peanut rhizosphere. Moreover, all tested PSB strains could be able to successfully mobilize P from inorganic P fractions (non-occluded Al-P and Fe-P). The strains CTC12 and KHD08 increased the pod yield (114 and 113%), shoot P (92 and 94%), and kernel P (100 and 101%), respectively, over the control. However, B. amyloliquefaciens CTC12 and B. cepacia KHD08 proved to be the potent P solubilizers in promoting peanut growth and yield.
本研究分析了来自奥里萨邦酸性土壤的天然磷细菌（PSB）菌株的体外磷（钙磷、铝磷、亚铁磷和铁（III）磷）溶解效率，以及通过生物施肥对花生（落花生）生长和磷吸收的利用情况。从奥里萨邦的五个地区，即巴拉索尔、库塔克、科德哈、基奥贾尔和梅奥尔班杰，收集了106份pH≤5.50的土壤样本。从每个地区选择一株细菌分离株，并在含有钙、铝和铁复合磷酸盐的国家植物学研究所磷酸盐肉汤中分析其磷溶解效率。在培养8天后，CTC12和KHD08从钙磷（CTC12为393.30mg/L；KHD08为465.25mg/L）、铝磷（CTC12为40.00mg/L；KHD08为34.50mg/L）、铁（III）磷（CTC12为175.50mg/L；KHD08为168.75mg/L）和亚铁磷（CTC12为47.40mg/L；KHD08为42.00mg/L）中转化出了更多的可溶性磷。在肉汤培养基中，所有五株菌株对磷的生物转化率顺序为钙磷>铁（III）磷>亚铁磷>铝磷。鉴定出的五株菌株分别为蜡样芽孢杆菌BLS18（KT582541）、解淀粉芽孢杆菌CTC12（KT633845）、洋葱伯克霍尔德菌KHD08（KT717633）、洋葱伯克霍尔德菌KJR03（KT717634）和洋葱伯克霍尔德菌K1（KM030037），并进一步研究了它们对花生的生物施肥效果。与未接种的对照盆相比，CTC12和KHD08分别使花生根际土壤有效磷增加了约65%和58%，并使每种铝的含量分别降低了约79%和81%。此外，所有测试的PSB菌株都能够成功地从无机磷组分（非闭蓄态铝磷和铁磷）中活化磷。与对照相比，菌株CTC12和KHD08分别使荚果产量提高了114%和113%，地上部磷含量提高了92%和94%，果仁磷含量提高了100%和101%。然而，解淀粉芽孢杆菌CTC12和洋葱伯克霍尔德菌KHD08被证明是促进花生生长和产量的有效磷溶解菌。
