Feng Li-Juan, Jia Rong, Sun Jing-Ya, Wang Jing, Lv Zheng-Hui, Mu Jun, Yang Guang-Feng
Department of Environmental Engineering, Zhejiang Ocean University, No.1 Haida South Road, Zhoushan, 316022, People's Republic of China.
Biodegradation. 2017 Aug;28(4):231-244. doi: 10.1007/s10532-017-9792-y. Epub 2017 Apr 28.
Understanding the dynamics of performance and bacterial community of biofilm under oligotrophic stress is necessary for the process optimization and risk management in biofilm systems for raw water pretreatment. In this study, biofilm obtained from a pilot-scale biofilm reactor was inoculated into a pilot-scale experimental tank for the treatment of oligotrophic raw water. Results showed that the removal of NH-N was impaired in biofilm systems when influent NH-N was less than 0.35 mg L or NH-N loading rate of less than 7.51 mg L day. The dominant bacteria detected in biofilm of different carrier were obvious distinct from phylum to genus level under oligotrophic stress. The dominant bacteria in elastic stereo media carrier changed from Proteobacteria (51.1%) to Firmicutes (32.7%), while Proteobacteria was always dominant in suspended ball carrier after long-term operation under oligotrophic conditions. Oligotrophic stress largely decreased the functional bacteria for the removal of nitrogen and organics including many genera in Proteobacteria and Nitrospirae, but increased several genera with spore forming organisms or potential bacterial pathogens in ESM carrier mainly including Bacillus, Mycobacterium, Pseudomonas, etc.
了解贫营养胁迫下生物膜的性能动态和细菌群落对于原水预处理生物膜系统的工艺优化和风险管理至关重要。在本研究中，将取自中试规模生物膜反应器的生物膜接种到中试规模的实验水箱中，用于处理贫营养原水。结果表明，当进水NH-N小于0.35 mg/L或NH-N负荷率小于7.51 mg/(L·d)时，生物膜系统中NH-N的去除受到损害。在贫营养胁迫下，不同载体生物膜中检测到的优势细菌在门到属水平上明显不同。弹性立体介质载体中的优势细菌从变形菌门（51.1%）变为厚壁菌门（32.7%），而在贫营养条件下长期运行后，悬浮球载体中的优势细菌始终是变形菌门。贫营养胁迫大大减少了包括变形菌门和硝化螺旋菌门中的许多属在内的用于去除氮和有机物的功能细菌，但增加了弹性立体介质载体中一些具有产孢生物或潜在细菌病原体的属，主要包括芽孢杆菌属、分枝杆菌属、假单胞菌属等。
