Rossowski W J, Ozden A, Ertan A, Maumus M, Arimura A
Dept. of Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Scand J Gastroenterol. 1988 Aug;23(6):717-25. doi: 10.3109/00365528809093939.
Clinical and experimental data indicate that the concentration of gastrin-I and somatostatin binding sites in human and rat gastric and duodenal mucosa may be changed in several pathologic conditions, including human peptic ulcer and cancer diseases. There are no data, however, indicating the distribution of receptor binding sites in the normal upper gastrointestinal tract. We studied the regional distribution of somatostatin-14, gastrin-I, and cholinergic muscarinic binding sites in membrane preparations from rat gastric corporeal and antral mucosa and in mucosa obtained from the duodenum and jejunum. The corporeal mucosa contained the most high-affinity gastrin binding sites (Bmax = 39.1 +/- 6.5 fmol/mg protein; Kd = 1.1 +/- 0.4 nM). The antral mucosa contained the most somatostatin and cholinergic muscarinic binding sites (Bmax = 65.7 +/- 6.6 fmol/mg protein and 460.3 +/- 101.8 fmol/mg protein, respectively). The duodenal and jejunal mucosal membranes contained somatostatin, gastrin, and cholinergic muscarinic binding sites in decreasing concentrations. Concentrations of binding sites are characteristic for particular gut regions and may help in analyzing their abnormalities.
临床和实验数据表明，在包括人类消化性溃疡和癌症疾病在内的几种病理状况下，人和大鼠胃及十二指肠黏膜中胃泌素 -I 和生长抑素结合位点的浓度可能会发生变化。然而，尚无数据表明正常上消化道中受体结合位点的分布情况。我们研究了大鼠胃体和胃窦黏膜以及十二指肠和空肠黏膜制备物中生长抑素 -14、胃泌素 -I 和胆碱能毒蕈碱结合位点的区域分布。胃体黏膜含有最高亲和力的胃泌素结合位点（Bmax = 39.1 ± 6.5 fmol/mg 蛋白；Kd = 1.1 ± 0.4 nM）。胃窦黏膜含有最多的生长抑素和胆碱能毒蕈碱结合位点（分别为 Bmax = 65.7 ± 6.6 fmol/mg 蛋白和 460.3 ± 101.8 fmol/mg 蛋白）。十二指肠和空肠黏膜膜中生长抑素、胃泌素和胆碱能毒蕈碱结合位点的浓度依次降低。结合位点的浓度是特定肠道区域的特征，可能有助于分析其异常情况。
