Harty R F, Maico D G, McGuigan J E
Gut. 1986 Jun;27(6):652-8. doi: 10.1136/gut.27.6.652.
Organ culture was used to compare gastrin and somatostatin release from cultured antral mucosa obtained from duodenal ulcer and non-ulcer (control) subjects. In response to dibutyryl cyclic AMP (DBCAMP) cultured antral mucosal explants from patients with a history of duodenal ulcer released a greater proportion of antral gastrin into the medium than did antral mucosal explants from non-ulcer subjects. Somatostatin release from antral mucosa from duodenal ulcer patients was substantially less than somatostatin released by antral explants from non-ulcer subjects. In the non-ulcer subjects there was a direct positive correlation between the amounts of antral somatostatin and gastrin released into the culture medium (r = 0.64, less than p 0.01). In the duodenal ulcer patients, however, there was no correlation between gastrin release and somatostatin release from antral mucosa ( r = 0.09; p greater than 0.2). Results of these studies identify enhanced gastrin release in response to stimulation and decreased release of somatostatin from antral mucosa of duodenal ulcer patients. These alterations in paracrine relationships of antral somatostatin and gastrin in duodenal ulcer subjects may contribute, at least in part, to the pathogenesis of duodenal ulcer disease.
采用器官培养法比较十二指肠溃疡患者和非溃疡（对照）患者的胃窦黏膜培养物中胃泌素和生长抑素的释放情况。对二丁酰环磷腺苷（DBCAMP）的反应中，有十二指肠溃疡病史患者的胃窦黏膜外植体向培养基中释放的胃窦胃泌素比例高于非溃疡患者的胃窦黏膜外植体。十二指肠溃疡患者胃窦黏膜释放的生长抑素明显少于非溃疡患者胃窦外植体释放的生长抑素。在非溃疡患者中，释放到培养基中的胃窦生长抑素量与胃泌素量之间存在直接正相关（r = 0.64，p<0.01）。然而，在十二指肠溃疡患者中，胃窦黏膜释放的胃泌素与生长抑素之间无相关性（r = 0.09；p>0.2）。这些研究结果表明，十二指肠溃疡患者的胃窦黏膜对刺激的反应增强，胃泌素释放增加，生长抑素释放减少。十二指肠溃疡患者胃窦生长抑素和胃泌素旁分泌关系的这些改变可能至少部分促成十二指肠溃疡病的发病机制。
