Department of Technical Textiles, College of Textiles, Donghua University, Shanghai, 201620, China.
Key Laboratory of Textile Science and Technology (Donghua University), Ministry of Education, Shanghai, 201620, China.
J Biomed Mater Res B Appl Biomater. 2018 Apr;106(3):954-964. doi: 10.1002/jbm.b.33909. Epub 2017 Apr 29.
Polyethylene glycol terephthalate (PET) fabrics with woven structures have proved to be quite effective for use on large diameter artificial blood vessels. However, their use within small-diameter artificial blood vessels has been associated with poor long-term patency, a problem resulting from slow endothelialization on PET and an over hyperplasia of smooth muscle cells. Previous research from our laboratory has revealed that ICAM-1 can be used as a marker to investigate cell adhesion, an effect which was closely associated with cell behavior on the surface of polycaprolactone (PCL) films. Moreover, we found that the coarseness or pore size of the surface exerts considerable influence on cell adhesion and proliferation on PCL films. In this study, we successfully fabricated six types of PET woven fabrics with varying gradients of tightness and porosities. Levels of ICAM-1 expression (membrane ICAM-1 & soluble ICAM-1) were then determined in these woven fabrics. Our results show that increased levels of mICAM-1 and decreased levels of sICAM-1 expression were obtained in HUVECs seeded on these six samples. These findings indicate that cell adhesion and proliferation on fabric surfaces were strongly influenced by their structural parameters, in particular the initial adhesion between the cell and fabric surface. In addition, we also found that extracellular matrix adhesion tends to prefer flat and tight surfaces, which promotes cell-cell and cell-matrix interactions, as well as the endothelialization on the surface of PET fabrics. These findings provide some novel insights with regard to the design and application of small-diameter artificial blood vessels. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 954-964, 2018.
聚对苯二甲酸乙二醇酯（PET）织物具有编织结构，已被证明在大直径人造血管中非常有效。然而，在小直径人造血管中的应用与较差的长期通畅率有关，这是由于 PET 内皮化缓慢和平滑肌细胞过度增生所致。我们实验室的先前研究表明，ICAM-1 可用作研究细胞黏附的标志物，这种作用与聚己内酯（PCL）薄膜表面上的细胞行为密切相关。此外，我们发现表面的粗糙度或孔径对 PCL 薄膜上的细胞黏附和增殖有很大影响。在这项研究中，我们成功地制造了六种具有不同紧密程度和孔隙率的 PET 机织织物。然后测定这些机织织物中 ICAM-1 的表达水平（膜 ICAM-1 和可溶性 ICAM-1）。我们的结果表明，在这些六种样本上接种的 HUVECs 中，mICAM-1 的水平增加，sICAM-1 的水平降低。这些发现表明，细胞在织物表面的黏附和增殖受到其结构参数的强烈影响，特别是细胞与织物表面之间的初始黏附。此外，我们还发现细胞外基质黏附倾向于平而紧密的表面，这促进了细胞-细胞和细胞-基质的相互作用，以及 PET 织物表面的内皮化。这些发现为小直径人造血管的设计和应用提供了一些新的见解。©2017Wiley 期刊，Inc. J Biomed Mater Res Part B: Appl Biomater，106B：954-964，2018。
