Department of textile engineering, Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.
Department of Immunology, Binzhou Medical College, Yantai, 264003, China.
J Biomed Mater Res A. 2016 Nov;104(11):2775-84. doi: 10.1002/jbm.a.35818. Epub 2016 Jul 14.
Here, we investigate the relationship between the expression of intercellular adhesion molecule-1 (ICAM-1) and the adhesion of human umbilical vein endothelial cells (HUVECs) on a poly-ε-caprolactone (PCL) film with micropores of different pore sizes. The results showed that surface hydrophilicity increased with larger pore sizes, while surfaces became less hydrophilic as the pore size decreased. The ability for adhesion and proliferation of HUVECs on surfaces with larger pore sizes was enhanced as compared with that of surfaces with smaller pore sizes or a flat film. Furthermore, levels of mICAM-1 were increased and sICAM-1 decreased as a function of increasing pore size. These findings demonstrate that film surfaces with larger pore sizes may promote cell adhesion and proliferation and lead to increases in expression of mICAM-1. Thus, we conclude that the pore size of the material's surface exerts a significant impact on the expression of adhesion molecules, the expression of which can represent an important new marker for investigating cell-surface adhesion and proliferation. Moreover, as elevated levels of sICAM-1 are associated with conditions such as inflammation, thrombosis, cerebral infarct and other diseases in vivo, it may serve as an early-warning risk marker when using medical biomaterials. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2775-2784, 2016.
在这里，我们研究了细胞间黏附分子-1（ICAM-1）的表达与不同孔径微孔聚己内酯（PCL）膜上人脐静脉内皮细胞（HUVEC）黏附之间的关系。结果表明，表面亲水性随孔径增大而增加，而随孔径减小表面亲水性降低。与小孔径或平整膜相比，大孔径表面上 HUVEC 的黏附和增殖能力增强。此外，随着孔径的增大，mICAM-1 的水平升高，sICAM-1 降低。这些发现表明，较大孔径的膜表面可能促进细胞黏附和增殖，并导致 mICAM-1 的表达增加。因此，我们得出结论，材料表面的孔径对黏附分子的表达有显著影响，其表达可以作为研究细胞表面黏附和增殖的一个重要新标志物。此外，由于 sICAM-1 的水平升高与体内炎症、血栓形成、脑梗死等疾病有关，因此在使用医用生物材料时，它可能作为一个预警风险标志物。© 2016 Wiley Periodicals, Inc. J 生物材料 A 部分：104A：2775-2784，2016。
