从杜氏盐藻中提高脂类或类胡萝卜素产量的氮磷补充策略。
Nitrogen or phosphorus repletion strategies for enhancing lipid or carotenoid production from Tetraselmis marina.
机构信息
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, PO Box «1177», 3018 Sfax, Tunisia.
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, PO Box «1177», 3018 Sfax, Tunisia.
出版信息
Bioresour Technol. 2017 Aug;238:325-332. doi: 10.1016/j.biortech.2017.04.008. Epub 2017 Apr 13.
The objective of this study was to investigate the accumulation of lipid and photosynthetic pigments from Tetraselmis marina. When the cells were grown in F/2-medium for seven days in the first stage, the carotenoid and lipid contents, and productivity were 44g/kg (DW), 27% and 31mg/L/d, respectively. After second stage of cultivation of T. marina for further 3-days under N-replete condition (4.41mM NaNO) increased biomass concentration of 1900mg/L and lipid content of 50% were observed, with an enhanced lipid productivity of 86.36mg/L/d and SFA and MUFA fractions of 70.76 and 13.14%, respectively. However, under P-repletion (2.08mM NaHPO), its carotenoid content increased to 89.23g/kg and its PUFA for 65% of total lipids. Results showed that N and P-replete conditions decreased SOD activity and increased HO and TBARS levels of T. marina. Thus, this native microalga strain could be a potent candidate for feed, food or biofuel production.
本研究旨在探究马尾藻（Tetraselmis marina）中脂类和光合色素的积累情况。当细胞在第一阶段的 F/2 培养基中生长七天时，类胡萝卜素和脂类的含量、生产率分别为 44g/kg（干重）、27%和 31mg/L/d。在第二阶段，当 T. marina 在氮充足条件下（4.41mM NaNO）进一步培养 3 天时，观察到生物量浓度增加到 1900mg/L，脂类含量增加到 50%，脂类生产率提高到 86.36mg/L/d，并且 SFA 和 MUFA 分别占 70.76%和 13.14%。然而，在磷充足条件下（2.08mM NaHPO），其类胡萝卜素含量增加到 89.23g/kg，多不饱和脂肪酸（PUFA）占总脂类的 65%。结果表明，氮和磷充足条件下降低了 SOD 活性，增加了 T. marina 的 HO 和 TBARS 水平。因此，这种本地微藻株可能是饲料、食品或生物燃料生产的潜在候选物。
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