Wan Liang, Zhou Yan, Huang Rong, Jiao Yiying, Gao Jian
Key Laboratory of Intelligent Health Perception and Ecological Restoration of Rivers and Lakes, Ministry of Education, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China.
Innovation Demonstration Base of Ecological Environment Geotechnical and Ecological Restoration of Rivers and Lakes, Hubei University of Technology, Wuhan 430068, China.
Toxics. 2024 Aug 20;12(8):611. doi: 10.3390/toxics12080611.
Moxifloxacin (MOX), a widely used novel antibiotic, may pose ecological risks at its actual environmental concentrations, as has been detected in aquatic systems. However, its ecotoxicity to aquatic organisms and regulatory mechanisms of phosphorus in eutrophic aqueous environments are still limited. This study aimed to analyze its physiological and biochemical parameters, including cellular growth, chlorophyll fluorescence, photosynthetic pigments, oxidative stress biomarkers, and metabolomics to elucidate the toxicity induced by environmental concentrations of MOX in at different phosphorus levels. The results revealed that the EC values of MOX on at different phosphorus concentrations were 8.03, 7.84, and 6.91 μg/L, respectively, indicating MOX toxicity was exacerbated with increasing phosphorus levels. High phosphorus intensified the suppression of chlorophyll fluorescence and photosynthetic pigments, while activating the antioxidant enzyme, indicating severe peroxidation damage. Metabolomic analysis showed MOX induced different discriminating metabolites under different phosphorus levels, and perturbed more biological pathways at higher phosphorus concentrations, such as starch and sucrose metabolism, pyrimidine metabolism, and glycerolipid metabolism. This indicates that phosphorus plays an important role in regulating metabolism in exposed to MOX. The findings provide valuable information on the mechanisms involved in cyanobacteria responses to antibiotic stress, and offer a theoretical basis for accurately assessing antibiotic toxicity in eutrophic aqueous environments.
莫西沙星（MOX）是一种广泛使用的新型抗生素，在水生系统中已检测到其实际环境浓度可能带来生态风险。然而，其对水生生物的生态毒性以及在富营养化水环境中对磷的调控机制仍较为有限。本研究旨在分析其生理生化参数，包括细胞生长、叶绿素荧光、光合色素、氧化应激生物标志物和代谢组学，以阐明不同磷水平下环境浓度的莫西沙星对[研究对象未明确给出，暂译为“该生物”]诱导的毒性。结果表明，不同磷浓度下莫西沙星对该生物的EC值分别为8.03、7.84和6.91μg/L，表明随着磷水平的升高，莫西沙星的毒性加剧。高磷加剧了对叶绿素荧光和光合色素的抑制，同时激活了抗氧化酶，表明存在严重的过氧化损伤。代谢组学分析表明，莫西沙星在不同磷水平下诱导出不同的差异代谢物，且在较高磷浓度下扰乱了更多的生物途径，如淀粉和蔗糖代谢、嘧啶代谢和甘油脂代谢。这表明磷在调节受莫西沙星影响的该生物的代谢中起重要作用。这些发现为蓝藻对抗生素胁迫的响应机制提供了有价值的信息，并为准确评估富营养化水环境中抗生素的毒性提供了理论依据。
