对不依赖自噬相关蛋白5的线粒体自噬的监测

Monitoring of Atg5-Independent Mitophagy.

作者信息

Arakawa Satoko, Honda Shinya, Torii Satoru, Tsujioka Masatsune, Shimizu Shigeomi

机构信息

Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.

出版信息

Methods Mol Biol. 2018;1759:125-132. doi: 10.1007/7651_2017_16.

DOI:10.1007/7651_2017_16

PMID:28456949

Abstract

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.

摘要

线粒体自噬是一种线粒体质量控制机制，受损和多余的线粒体通过自噬被清除。哺乳动物至少有两种不同的自噬途径：依赖Atg5的传统途径和不依赖Atg5的替代途径；后者参与红细胞的线粒体自噬。在本章中，我们描述了评估哺乳动物细胞中不依赖Atg5的线粒体自噬的各种实验方法。