Adur Malavika K, Hale Benjamin J, Ross Jason W
Department of Animal Science, Iowa State University, 2356 Kildee Hall, Ames, IA, 50011, USA.
Methods Mol Biol. 2017;1605:63-81. doi: 10.1007/978-1-4939-6988-3_5.
MicroRNAs (miRNAs) play a crucial role in the regulation of many post-transcriptional processes in reproductive cells. Regulation of maternal mRNA translation and activation of zygotic mRNA are essential to successful embryonic development. Moreover, the precise development of embryonic cell and/or tissue lineages requires temporal and spatial control of gene expression, mRNA abundance, and translation into proteins, which is in part regulated via miRNA. Here, we describe some key protocols that can be utilized to detect and quantify miRNA in in vitro produced oocytes and embryos.
微小RNA（miRNA）在生殖细胞中许多转录后过程的调控中发挥着关键作用。母体mRNA翻译的调控和合子mRNA的激活对于胚胎的成功发育至关重要。此外，胚胎细胞和/或组织谱系的精确发育需要对基因表达、mRNA丰度以及翻译成蛋白质进行时空控制，这部分是通过miRNA来调控的。在此，我们描述了一些可用于检测和定量体外产生的卵母细胞和胚胎中miRNA的关键方案。
