通过流式细胞术检测血小板中的溶酶体胞吐作用。

Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry.

作者信息

Södergren Anna L, Ramström Sofia

机构信息

Clinical Chemistry, Department of Clinical and Experimental Medicine, Linköping University, SE-58185, Linköping, Sweden.

Department of Clinical Chemistry and Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.

出版信息

Methods Mol Biol. 2017;1594:191-203. doi: 10.1007/978-1-4939-6934-0_12.

DOI:10.1007/978-1-4939-6934-0_12

PMID:28456984

Abstract

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

摘要

流式细胞术是一种能够对悬浮液中的单个细胞进行高通量分析的方法。通过加入荧光标记抗体，无需事先裂解细胞就可以分析一种或多种表面抗原（如溶酶体相关膜蛋白1，即LAMP-1）的丰度。在此，我们描述了用流式细胞术分析血小板溶酶体胞吐作用时所需的特殊注意事项。