Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, PR China.
Carbohydr Polym. 2017 Jul 15;168:103-111. doi: 10.1016/j.carbpol.2017.02.036. Epub 2017 Feb 14.
Cyclodextrin-based supermolecular systems have become one of significant nonviral gene delivery carriers. In this study, epsilon-polylysine-grafted-succinic acid-grafted-β-cyclodextrin-LMW PEI (PPC) and adamantane-functionalized poly-(ethylene glycol) derivative (PEG-AD) were synthesized, and PEG-AD was encapsulated into PPC to form the complexes. These complexes were used to condense pDNA to make polyplexes, which biophysical properties, cytotoxicity and transfection efficiencies were studied. The results showed that the polyplexes were less cytotoxic than branched PEI without degrading the transfection efficiency. These findings suggest that the complexes with high stability could be an effective and low-toxicity carrier for delivering nucleic acid to target cells.
基于环糊精的超分子体系已成为重要的非病毒基因传递载体之一。本研究合成了ε-聚赖氨酸接枝琥珀酸接枝-β-环糊精-低分子量聚乙烯亚胺（PPC）和金刚烷功能化聚（乙二醇）衍生物（PEG-AD），并将 PEG-AD 包封到 PPC 中形成复合物。这些复合物被用来凝聚 pDNA 以形成聚阳离子复合物，研究了它们的物理化学性质、细胞毒性和转染效率。结果表明，这些聚阳离子复合物的细胞毒性低于支化聚乙烯亚胺，而转染效率没有降低。这些发现表明，具有高稳定性的复合物可能是一种有效且低毒的载体，可将核酸递送到靶细胞。
