Kanawaku Yoshimasa, Hirakawa Keiko, Koike Kaoru, Kanetake Jun, Ohno Youkichi
Department of Legal Medicine, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo 113-8602, Japan.
Department of Legal Medicine, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo 113-8602, Japan.
Leg Med (Tokyo). 2017 Mar;25:52-58. doi: 10.1016/j.legalmed.2017.01.004. Epub 2017 Jan 16.
Cerebrospinal fluid (CSF) is routinely subjected to gross evaluation in postmortem investigations; however, its use in chemical evaluations has not been fully realized. Analysis of nuclear magnetic resonance (NMR) spectra with pattern recognition methods was applied to CSF samples. Rats were treated with pentylenetetrazol (PTZ) to induce seizure or pentobarbital (PB) to induce coma, and postmortem CSF was collected after CO gas euthanization. Pattern recognition analysis of the NMR data was performed on individual postmortem CSF samples. The aim of this study was to determine if pattern recognition analysis of NMR data could be used to classify the rats according to their drug treatment. The applicability of NMR data with pattern recognition analysis using postmortem CSF was also assessed. Partial Least Squares-Discriminant Analysis (PLS-DA) score plots indicated that the PTZ, PB, and NS (control) groups were clustered and clearly separated. PLS-DA correlation loading plots showed respective spectral and category variances of 41% and 42% for factor 1, and 17% and 27% for factor 2. Thus, factors 1 and 2 together described 58% (41%+17%) and 69% (42%+27%) of the variation, respectively. NMR study of postmortem CSF has the potential to be utilized as both a novel forensic neurochemistry method and in the clinical setting.
在尸检调查中，脑脊液（CSF）通常会进行大体评估；然而，其在化学评估中的应用尚未得到充分实现。将模式识别方法应用于脑脊液样本的核磁共振（NMR）光谱分析。用戊四氮（PTZ）诱导大鼠癫痫发作，或用戊巴比妥（PB）诱导大鼠昏迷，在一氧化碳气体安乐死后收集死后脑脊液。对单个死后脑脊液样本进行核磁共振数据的模式识别分析。本研究的目的是确定核磁共振数据的模式识别分析是否可用于根据大鼠的药物治疗情况对其进行分类。还评估了使用死后脑脊液进行模式识别分析的核磁共振数据的适用性。偏最小二乘判别分析（PLS-DA）得分图表明，PTZ组、PB组和NS（对照组）聚集在一起且明显分开。PLS-DA相关载荷图显示，因子1的光谱方差和类别方差分别为41%和42%，因子2的光谱方差和类别方差分别为17%和27%。因此，因子1和因子2共同分别描述了58%（41%+17%）和69%（42%+27%）的变异。死后脑脊液的核磁共振研究有潜力既作为一种新型法医神经化学方法，也用于临床环境。
