Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
Clin Nutr. 2018 Jun;37(3):965-969. doi: 10.1016/j.clnu.2017.04.001. Epub 2017 Apr 9.
It has been reported that people may respond differently to the same environmental changes because of genome variations.
The main purpose of the present study is to determine gene-diet interactions between -265T>C apolipoprotein A-II polymorphisms and evaluate the effect of weight loss on parameters related to HDL function.
In the present study, 56 overweight and obese type 2 diabetic patients were chosen from 697 genotype-specified subjects. After matching for gender, age and BMI, an equal number of patients were chosen for each genotype of APOA-II (TT/TC and CC group). After six-week calorie restriction programme, 44 patients completed the study. Serum paraoxonase-1 (PON1), paraoxonase-3 (PON3), pentraxin-3 (PTX3), and PTX3 gene expression in peripheral blood mononuclear cells were compared between two genotypes and also before and after the intervention separated in each genotype.
The mean differences of PON enzymes and PTX3 between groups were not significant at the baseline. After weight loss, the mean weight, BMI and serum concentration of PON1 and PON3 decreased significantly and PTX3 increased in total population. Although, the mean differences of PON enzymes and PTX3 between two groups were not significant. However, in comparison of mean differences within the groups, decreased PON3 and increased PTX3 have been observed only in TT group.
A comparison of the mean differences in PON3 and PTX3 within two genotype groups showed that T allele carriers are more sensitive to lifestyle modification, and serum PON3 and PTX3 levels significantly changed only in the TT/TC group.
据报道，由于基因组变异，人们可能会对相同的环境变化做出不同的反应。
本研究的主要目的是确定载脂蛋白 A-II-265T>C 多态性与饮食的基因-饮食相互作用，并评估减肥对与高密度脂蛋白功能相关参数的影响。
本研究从 697 名基因型指定的受试者中选择了 56 名超重和肥胖的 2 型糖尿病患者。在性别、年龄和 BMI 匹配后，为每种 APOA-II 基因型（TT/TC 和 CC 组）选择相同数量的患者。经过 6 周的热量限制计划后，44 名患者完成了研究。比较了两组之间以及在每种基因型中分别在干预前后外周血单核细胞中对氧磷酶-1（PON1）、对氧磷酶-3（PON3）、五聚素-3（PTX3）和 PTX3 基因表达的差异。
在基线时，两组之间 PON 酶和 PTX3 的平均差异不显著。减肥后，总人群的体重、BMI 和 PON1 和 PON3 的血清浓度显著降低，PTX3 增加。尽管两组之间 PON 酶和 PTX3 的平均差异不显著，但在组内平均差异的比较中，仅在 TT 组观察到 PON3 降低和 PTX3 升高。
对两组基因型内 PON3 和 PTX3 的平均差异进行比较表明，T 等位基因携带者对生活方式改变更敏感，只有 TT/TC 组的血清 PON3 和 PTX3 水平显著改变。
