Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, International Campus, Tehran University of Medical Sciences, Hojatdoost Ave., Naderi St., Keshavarz Blvd., Tehran, Iran.
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
Nutr J. 2017 May 25;16(1):34. doi: 10.1186/s12937-017-0255-4.
People may have different responses to the same environmental changes. It has been reported that genome variations may be responsible for these differences. Also, HDL subfractions may be influenced by different genetic variations. The aim of the present study was to determine gene-diet interactions and to evaluate the influence of weight loss on HDL subfractions between two genotypes of -265 T>C APOA-II polymorphism.
In the present study, 56 overweight and obese patients with type 2 diabetes mellitus were selected from 697 genotype-specified subjects. After matching for gender, age and BMI at the beginning of the study, an equal number of patients remained on each genotype of APOA-II (TT/TC and CC group). After a 6-week calorie restriction program, 44 patients completed the study. Serum HDL subfractions, including HDL2 and HDL3 and LCAT activity, were compared between the two genotypes and, before and after the intervention, were separated in each genotype.
Serum concentration of HDL and its subfractions decreased significantly due to the weight loss. A comparison of the mean changes between the genotypes showed that HDL3 significantly decreased in the CC genotype while, in the TT/TC group, the serum concentration of HDL2 was significantly reduced. However, the increase of LCAT activity was not significant among the two genotypes.
A comparison of mean changes of variables within two genotype groups showed that C homozygote carriers lead to a general shift toward larger size HDL subfractions and T allele carriers shift toward smaller size HDL subfractions after weight loss.
人们可能对相同的环境变化有不同的反应。据报道，基因组变异可能是造成这些差异的原因。此外，HDL 亚组分可能受到不同遗传变异的影响。本研究旨在确定基因-饮食相互作用，并评估两种载脂蛋白 A-II -265T>C 多态性基因型个体之间体重减轻对 HDL 亚组分的影响。
本研究从 697 名基因型指定的受试者中选择了 56 名超重和肥胖的 2 型糖尿病患者。在研究开始时，根据性别、年龄和 BMI 进行匹配后，每种载脂蛋白 A-II 基因型（TT/TC 和 CC 组）都有相同数量的患者。经过 6 周的热量限制计划后，有 44 名患者完成了研究。比较两种基因型之间的血清 HDL 亚组分，包括 HDL2 和 HDL3 以及 LCAT 活性，并在干预前后，按基因型进行分组。
由于体重减轻，血清 HDL 及其亚组分的浓度显著降低。比较两种基因型之间的平均变化，CC 基因型的 HDL3 显著降低，而 TT/TC 组的 HDL2 血清浓度显著降低。然而，两种基因型之间 LCAT 活性的增加并不显著。
对两个基因型组内变量的平均变化进行比较，结果表明 C 纯合子携带者导致 HDL 亚组分向较大粒径转移，而 T 等位基因携带者在体重减轻后向较小粒径 HDL 亚组分转移。
