Haghighat Setareh, Siadat Seyed Davar, Rezayat Sorkhabadi Seyed Mehdi, Akhavan Sepahi Abbas, Sadat Seyed Mehdi, Yazdi Mohammad Hossein, Mahdavi Mehdi
Department of Microbiology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Department of Mycobacteriology & Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Microb Pathog. 2017 Jul;108:32-39. doi: 10.1016/j.micpath.2017.04.037. Epub 2017 Apr 28.
Methicillin-resistant Staphylococcus aureus infections are focal and development of an effective vaccine can help to control this infection. Here, recombinant PBP2a was studied in mouse model. Following the preparation of recombinant PBP2a, Balb/c mice were injected subcutaneously with 20 μg of r-PBP2a formulated in Freund's adjuvant three times with three weeks intervals with proper control group. Total and specific isotype antibodies were evaluated on sera by ELISA. Opsonophagocytic activity was also investigated on the sera samples. Intraperitonealchallenge with a sub-lethal dose of MRSA (5 × 10 CFU) was done in experimental mice. Following that, the number of bacteria from kidneys of experimental mice were determined. Survival rate was recorded for 60 days. Significant increase of antibody with high level of IgG1, IgG2a and IgG2b isotypes was demonstrated in vaccinated mice versus the control group (P < 0.005). The bacterial load in the kidneys from immunized mice was 1000 times less thancontrol group (PBS) and opsonophagocytic activity of immunized mice sera significantly increased (P < 0.0001). Finally the life span of immunized mice after bacterial challenge was extended versus control mice. These results may indicate the capacity of PBP2a as a candidate vaccine to control the MRSA infections.
耐甲氧西林金黄色葡萄球菌感染具有局限性，开发有效的疫苗有助于控制这种感染。在此，在小鼠模型中对重组PBP2a进行了研究。制备重组PBP2a后，将Balb/c小鼠皮下注射20μg用弗氏佐剂配制的r-PBP2a，共注射三次，间隔三周，并设置适当的对照组。通过ELISA评估血清中的总抗体和特异性同种型抗体。还对血清样本进行了调理吞噬活性研究。对实验小鼠进行腹腔注射亚致死剂量的耐甲氧西林金黄色葡萄球菌（5×10 CFU）。随后，测定实验小鼠肾脏中的细菌数量。记录60天的存活率。与对照组相比，接种疫苗的小鼠体内IgG1、IgG2a和IgG2b同种型抗体水平显著升高（P<0.005）。免疫小鼠肾脏中的细菌载量比对照组（PBS）少1000倍，免疫小鼠血清的调理吞噬活性显著增加（P<0.0001）。最后，与对照小鼠相比，细菌攻击后免疫小鼠的寿命延长。这些结果可能表明PBP2a作为候选疫苗控制耐甲氧西林金黄色葡萄球菌感染的能力。
