评估用于检测呼吸道样本中人博卡病毒的NS1基因特异性实时定量TaqMan聚合酶链反应
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples.
作者信息
Ligozzi Marco, Diani Erica, Lissandrini Federica, Mainardi Rosanna, Gibellini Davide
机构信息
Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada delle Grazie 8, 37134 Verona, Italy.
Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada delle Grazie 8, 37134 Verona, Italy.
出版信息
Mol Cell Probes. 2017 Aug;34:53-55. doi: 10.1016/j.mcp.2017.04.005. Epub 2017 Apr 27.
Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 10 and 9.4 × 10 copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.
人博卡病毒（HBoV）与呼吸道疾病有关。在此，我们评估了一种基于TaqMan的聚合酶链反应（PCR），用于检测所有四种HBoV亚型感染，其灵敏度高达每反应15个拷贝。为了在临床样本上评估该检测方法，我们分析了178例儿科病例的鼻咽抽吸物样本，在178例患者中的13例检测到HBoV基因组，病毒载量范围在1.6×10至9.4×10拷贝/毫升之间。这些结果表明，该方法可作为诊断HBoV感染的替代技术。
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