Midilli Kenan, Yılmaz Gülden, Türkoğlu Salih, Iskanova Baarnisa, Ergin Sevgi, Yarımcam Filiz, Ozdamar Melda, Gürol Yeşim, Taştan Yücel, Altaş Kemal
İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye.
Mikrobiyol Bul. 2010 Jul;44(3):405-13.
Human bocavirus (HBoV) which was described in 2005 by molecular techniques, is a member of Parvoviridae. The role of HBoV is being questioned in acute respiratory diseases (ARD) in many recent studies. The aim of this study was to determine the presence of HBoV DNA in the respiratory specimens of patients with ARD. A total of 155 throat swab and/or washing specimens from 76 children and 79 adults with ARD were examined. HBoV DNA was investigated by single step in-house polymerase chain reaction (PCR) using NS1 primers (5-'TATGGCCAAGGCAATCGTCCAAG-3', 5'-GCC GCGTGAACATGAGAAA-CAG-3') which amplify the 290 base pair region of NS1 gene located between nucleotides 1545-1835 of prototype HBoV st1 strain. HBoV DNA was detected in 5 (6.5%) of 76 children and 2 (2.5%) of 79 adults. Three sequenced samples showed 100% homology with the reference sequences. This study in which HBoV DNA was detected in children and adults with ARD, is the first HBoV prevalence study in Turkey. Larger scale prospective clinical and molecular studies are required to explain the association between HBoV and respiratory disease.
人博卡病毒（HBoV）于2005年通过分子技术被发现，是细小病毒科的成员。在最近的许多研究中，HBoV在急性呼吸道疾病（ARD）中的作用受到质疑。本研究的目的是确定ARD患者呼吸道标本中HBoV DNA的存在情况。共检测了76名儿童和79名成人ARD患者的155份咽拭子和/或冲洗标本。使用NS1引物（5'-TATGGCCAAGGCAATCGTCCAAG-3'，5'-GCC GCGTGAACATGAGAAA-CAG-3'）通过单步内部聚合酶链反应（PCR）检测HBoV DNA，该引物可扩增位于原型HBoV st1株核苷酸1545 - 1835之间的NS1基因的290个碱基对区域。在76名儿童中有5名（6.5%）和79名成人中有2名（2.5%）检测到HBoV DNA。三个测序样本与参考序列显示出100%的同源性。本研究在ARD儿童和成人中检测到HBoV DNA，是土耳其首次进行的HBoV患病率研究。需要更大规模的前瞻性临床和分子研究来解释HBoV与呼吸道疾病之间的关联。
Zotero 插件