Kumar Ranjeet, Rao D D, Dubla Rupali, Yadav J R
Health Physics Laboratory, GSO Complex, BARC, Tarapur 401504, India.
Radiation Safety Systems Division, BARC, Trombay, Mumbai 400085, India.
Appl Radiat Isot. 2017 Jul;125:176-179. doi: 10.1016/j.apradiso.2017.04.025. Epub 2017 Apr 20.
The conventional radio-analytical technique used for estimation of Pu-isotopes in urine samples involves anion exchange/TEVA column separation followed by alpha spectrometry. This sequence of analysis consumes nearly 3-4 days for completion. Many a times excreta analysis results are required urgently, particularly under repeat and incidental/emergency situations. Therefore, there is need to reduce the analysis time for the estimation of Pu-isotopes in bioassay samples. This paper gives the details of standardization of a rapid method for estimation of Pu-isotopes in urine samples using multi-purpose centrifuge, TEVA resin followed by alpha spectrometry. The rapid method involves oxidation of urine samples, co-precipitation of plutonium along with calcium phosphate followed by sample preparation using high volume centrifuge and separation of Pu using TEVA resin. Pu-fraction was electrodeposited and activity estimated using Pu tracer recovery by alpha spectrometry. Ten routine urine samples of radiation workers were analyzed and consistent radiochemical tracer recovery was obtained in the range 47-88% with a mean and standard deviation of 64.4% and 11.3% respectively. With this newly standardized technique, the whole analytical procedure is completed within 9h (one working day hour).
用于估算尿样中钚同位素的传统放射分析技术包括阴离子交换/TEVA柱分离，随后进行α能谱分析。这一分析流程完成需要近3至4天时间。很多时候，排泄物分析结果需要紧急得出，尤其是在重复检测以及偶然/紧急情况下。因此，有必要缩短生物检测样品中钚同位素估算的分析时间。本文详细介绍了一种使用多功能离心机、TEVA树脂随后进行α能谱分析来估算尿样中钚同位素的快速方法的标准化过程。该快速方法包括尿样氧化、钚与磷酸钙共沉淀，随后使用大容量离心机进行样品制备以及用TEVA树脂分离钚。钚组分进行电沉积，并通过α能谱分析使用钚示踪剂回收率估算活度。对10名辐射工作人员的常规尿样进行了分析，获得的放射化学示踪剂回收率一致，在47%至88%之间，平均值和标准差分别为64.4%和11.3%。采用这种新标准化的技术，整个分析过程可在9小时（一个工作日时长）内完成。
