Morad M, Davies N W, Ulrich G, Schultheiss H P
Department of Physiology, University of Pennsylvania, Philadelphia 19104.
Am J Physiol. 1988 Oct;255(4 Pt 2):H960-4. doi: 10.1152/ajpheart.1988.255.4.H960.
Antibodies previously described to inhibit specifically nucleotide transport (ADP-ATP carrier) of the inner mitochondrial membrane were found to bind specifically to the sarcolemma of the enzymatically isolated rat ventricular myocytes. In this communication, we report for the first time that a component of these antibodies enhanced the Ca2+ current in isolated cardiac myocytes and potentiated twitch tension in ventricular strips. Prolonged exposure of rat myocytes to large concentrations of antibodies caused spontaneous contractions, progressive cell deterioration, and death. Our results thus show that a component of antibodies against ADP-ATP carrier cross-reacts with cardiac sarcolemmal proteins enhancing the Ca2+ channel.
先前已描述过的能特异性抑制线粒体内膜核苷酸转运（ADP - ATP载体）的抗体，被发现能特异性结合酶解分离的大鼠心室肌细胞的肌膜。在本通讯中，我们首次报道这些抗体的一个组分可增强分离的心肌细胞中的Ca2+电流，并增强心室肌条的收缩张力。将大鼠心肌细胞长时间暴露于高浓度抗体中会导致自发收缩、细胞逐渐恶化并死亡。因此，我们的结果表明，抗ADP - ATP载体抗体的一个组分与心肌肌膜蛋白发生交叉反应，增强了Ca2+通道。
