Hennessy D P, Stelmasiak T, Johnston N E, Jackson P N, Outch K H
Department of Agriculture and Rural Affairs, Veterinary Research Institute, Attwood, Victoria, Australia.
Am J Vet Res. 1988 Aug;49(8):1276-83.
The effect of ACTH administration on plasma cortisol concentrations in purebred and crossbred pigs was investigated. Pigs were given either 25 IU of ACTH or physiologic saline solution IM. Blood samples were collected immediately before and 1 hour after ACTH or saline solution administration. Administration of ACTH resulted in a significant (P less than 0.01) increase in plasma cortisol concentration compared with that resulting from administration of saline solution; mean values after ACTH administration were similar in both breed groups. In contrast, a fivefold range of differences was observed among individual pigs of the same age, sex, and body weight, irrespective of breed group. The type and magnitude of the adrenocortical response was consistent and repeatable in pigs over a 3-month period, suggesting that pigs have a consistent capacity for adrenocortical response to ACTH administration. Development of a dynamic test allowed the high and low responding extremes in a population to be detected. The most suitable dose of synthetic ACTH was established to be 50 IU, and the best time for blood sample collection was 60 minutes after ACTH administration. The classification of individual pigs as high or low responders was repeatable and was not affected by prior short-term exposure to ACTH.
研究了促肾上腺皮质激素（ACTH）给药对纯种猪和杂交猪血浆皮质醇浓度的影响。给猪肌肉注射25国际单位的ACTH或生理盐溶液。在注射ACTH或盐溶液之前和之后1小时立即采集血样。与注射盐溶液相比，注射ACTH导致血浆皮质醇浓度显著升高（P<0.01）；两个品种组注射ACTH后的平均值相似。相比之下，在相同年龄、性别和体重的个体猪之间观察到了五倍的差异范围，无论品种组如何。在3个月的时间里，猪的肾上腺皮质反应类型和幅度是一致且可重复的，这表明猪对ACTH给药具有一致的肾上腺皮质反应能力。动态试验的开发使得能够检测群体中反应高和低的极端个体。确定合成ACTH的最合适剂量为50国际单位，采集血样的最佳时间是注射ACTH后60分钟。将个体猪分类为高反应者或低反应者是可重复的，并且不受先前短期接触ACTH的影响。
