Pedro Fábio Luis Miranda, Costa Laura Maria Amorim Santana, Filho Gilberto Siebert, Guedes Orlando Aguirre, Pereira Thiago Machado, Borges Alvaro Henrique
Department of Oral Sciences, University of Cuiabá, Cuiabá-MT, Brazil.
Open Dent J. 2017 Mar 22;11:133-139. doi: 10.2174/1874210601711010133. eCollection 2017.
The main goal of endodontic treatment is to achieve cleaning and shaping prior to the filling process.
This study aimed to evaluate, using atomic absorption spectrometry, the release of Calcium ions after the use of different chelating agents and protocols of agitation.
Ninety human canine teeth were randomly assigned to one of nine groups (n=10), as follows: 1) 0.2% Chitosan and manual agitation; 2) 0.2% Chitosan and sonic agitation; 3) 0.2% Chitosan and ultrasonic agitation; 4) 17% EDTA and manual agitation; 5) 17% EDTA and sonic agitation; 6) 17% EDTA and ultrasonic agitation; 7) distilled water and manual agitation; 8) distilled water and sonic agitation; 9) distilled water and ultrasonic agitation. Following instrumentation, all chelating substances remained inside the root canal for 3 min. Then the fluid was collected for the identification and quantification of Calcium ions. The amount of Calcium ions released in each group was compared using analysis of variance (ANOVA) and the Kolmogorov-Smirnov and Levene tests followed by Tukey's post-hoc test. Significance was set at 5%.
The groups in which 0.2% Chitosan was used showed the highest concentration of Calcium ions (p<0.05). Concerning the agitation method, ultrasonic agitation showed the greatest values, followed by sonic and manual agitation (all comparisons, p<0.05).
The present findings suggest that, among the combinations here tested, Chitosan associated with ultrasonic agitation yielded the greatest release of Calcium ions.
根管治疗的主要目标是在充填过程之前实现根管的清理和塑形。
本研究旨在使用原子吸收光谱法评估在使用不同螯合剂和搅拌方案后钙离子的释放情况。
90颗人犬牙被随机分为9组（n = 10），如下：1）0.2%壳聚糖与手动搅拌；2）0.2%壳聚糖与声波搅拌；3）0.2%壳聚糖与超声搅拌；4）17%乙二胺四乙酸（EDTA）与手动搅拌；5）17% EDTA与声波搅拌；6）17% EDTA与超声搅拌；7）蒸馏水与手动搅拌；8）蒸馏水与声波搅拌；9）蒸馏水与超声搅拌。根管预备后，所有螯合剂在根管内保留3分钟。然后收集液体以鉴定和定量钙离子。使用方差分析（ANOVA）以及Kolmogorov-Smirnov和Levene检验，随后进行Tukey事后检验，比较每组释放的钙离子量。显著性设定为5%。
使用0.2%壳聚糖的组显示出最高浓度的钙离子（p<0.05）。关于搅拌方法，超声搅拌显示出最高值，其次是声波搅拌和手动搅拌（所有比较，p<0.05）。
本研究结果表明，在本研究测试的组合中，壳聚糖与超声搅拌相结合产生了最大量的钙离子释放。
