Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health of Russian Federation, Gamaleya Street 18, Moscow 123098, Russia.
Biomed Res Int. 2017;2017:3865802. doi: 10.1155/2017/3865802. Epub 2017 Mar 26.
imposes serious health problems and causes infertility. Because of asymptomatic onset, it often escapes antibiotic treatment. Therefore, vaccines offer a better option for the prevention of unwanted inflammatory sequelae. The existence of serologically distinct serovars of suggests that a vaccine will need to provide protection against multiple serovars. spp. use a highly conserved type III secretion system (T3SS) composed of structural and effector proteins which is an essential virulence factor. In this study, we expressed the T3SS needle protein of TC_0037, an ortholog of CdsF, in a replication-defective adenoviral vector (AdTC_0037) and evaluated its protective efficacy in an intravaginal model. For better immune responses, we employed a heterologous prime-boost immunization protocol in which mice were intranasally primed with AdTC_0037 and subcutaneously boosted with recombinant TC_0037 and Toll-like receptor 4 agonist monophosphoryl lipid A mixed in a squalene nanoscale emulsion. We found that immunization with TC_0037 antigen induced specific humoral and T cell responses, decreased loads in the genital tract, and abrogated pathology of upper genital organs. Together, our results suggest that TC_0037, a highly conserved chlamydial T3SS protein, is a good candidate for inclusion in a vaccine.
它会导致严重的健康问题和不孕。由于无症状发病，它常常逃避抗生素治疗。因此，疫苗是预防不必要的炎症后遗症的更好选择。存在血清学上不同血清型的 表明疫苗将需要针对多种血清型提供保护。 spp. 使用高度保守的 III 型分泌系统（T3SS），由结构和效应蛋白组成，这是一种重要的毒力因子。在这项研究中，我们在复制缺陷型腺病毒载体（AdTC_0037）中表达了 TC_0037 的 T3SS 针蛋白，这是 CdsF 的同源物，并在阴道内 模型中评估了其保护效力。为了获得更好的免疫反应，我们采用了异源初免-加强免疫方案，即用 AdTC_0037 对小鼠进行鼻腔内初免，并用重组 TC_0037 和 Toll 样受体 4 激动剂单磷酰脂质 A 混合在角鲨烯纳米乳液中进行皮下加强免疫。我们发现，TC_0037 抗原免疫诱导了特异性体液和 T 细胞反应，降低了生殖道中的 负荷，并消除了上生殖道的病理学。总之，我们的结果表明，TC_0037 是一种高度保守的衣原体 T3SS 蛋白，是包含在 疫苗中的良好候选物。
