Zarate Ana Maria, Don Julieta, Secchi Dante, Carrica Andres, Galindez Costa Fernanda, Panico Rene, Brusa Martin, Barra José Luis, Brunotto Mabel
1 Departamento de Biología Bucal, Facultad de Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina.
2 Departamento de Patología Bucal, Facultad de Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina.
Tumour Biol. 2017 May;39(5):1010428317699113. doi: 10.1177/1010428317699113.
The aim of this work was to evaluate the prevalence of TP53Arg72Pro mutations and their possible relationship with oral carcinoma and oral potentially malignant disorders in Argentine patients. A cross-sectional study was performed on 111 exfoliated cytologies from patients with oral cancer (OC), oral potentially malignant disorders (OPMD) and controls. The TP53Arg72Pro mutations were determined using conventional PCR. We evaluated univariate and multivariate study variables, setting p < 0.05. We found: (a) a low frequency of Pro72 variant in control group and a high frequency in OC and OPMD, as well in OC and oral leukoplakia (OL) diagnosis; (b) multivariate association among the TP53CC genotype and females over 45 years with no tobacco nor alcohol habits with oral lichen planus pathology; (c) multivariate association between the TP53GC genotype and males with alcohol and tobacco habits and OC and OL pathologies. Our results showed that the wild-type Arg72variant was related to control patients and Pro72variant was related to OC and OPMD, in Argentine patients.
这项研究的目的是评估阿根廷患者中TP53Arg72Pro突变的患病率及其与口腔癌和口腔潜在恶性疾病的可能关系。对111例口腔癌（OC）、口腔潜在恶性疾病（OPMD）患者及对照者的脱落细胞学样本进行了横断面研究。采用常规PCR法检测TP53Arg72Pro突变。我们评估了单变量和多变量研究变量，设定p<0.05。我们发现：（a）对照组中Pro72变体的频率较低，而在OC和OPMD以及OC和口腔白斑（OL）诊断中的频率较高；（b）TP53CC基因型与45岁以上无吸烟和饮酒习惯的女性与口腔扁平苔藓病理之间存在多变量关联；（c）TP53GC基因型与有吸烟和饮酒习惯的男性以及OC和OL病理之间存在多变量关联。我们的结果表明，在阿根廷患者中，野生型Arg72变体与对照患者相关，而Pro72变体与OC和OPMD相关。
