Gao Shuai, Gao Hong, Fan Yuchen, Zhang Guanghua, Sun Fengkai, Zhao Jing, Li Feng, Yang Yang, Wang Kai
J Tradit Chin Med. 2016 Aug;36(4):464-70. doi: 10.1016/s0254-6272(16)30063-2.
To investigate the effect of Yiguanjian (YGJ) cataplasm on the development of opioid dependence in a mouse model of naloxone-induced opioid withdrawal syndrome.
One hundred Swiss albino mice, of equal male to female ratio, were randomly and equally divided into 10 groups. A portion (3 cm2) of the backside hair of the mice was removed 1 day prior to the experiment. Morphine (5 mg/kg) was intraperitoneally administered twice daily for 5 days. YGJ cataplasm was prepared and pasted on the bare region of the mice immediately before morphine administration on day 3 and subsequently removed at the end day 5. On day 6, naloxone (8 mg/kg) was intraperitoneally injected to precipitate opioid withdrawal syndrome. Behavioral observation was performed in two 30-min phases immediately after naloxone injection.
The YGJ cataplasm significantly and dose-dependently attenuated morphine-naloxone- induced experimental opioid withdrawal, in terms of withdrawal severity score and the frequencies of jumping, rearing, forepaw licking, and circling behaviors. However, YGJ cataplasm treatment did not alter the acute analgesic effect of morphine.
YGJ cataplasm could attenuate opioid dependence and its associated withdrawal symptoms. Therefore, YGJ cataplasm could serve as a potential therapy for opioid addiction in the future.
在纳洛酮诱发的阿片类药物戒断综合征小鼠模型中，研究一贯煎（YGJ）巴布剂对阿片类药物依赖性发展的影响。
100只瑞士白化小鼠，雌雄比例相等，随机等分为10组。实验前1天，去除小鼠背部一部分（3平方厘米）毛发。每天两次腹腔注射吗啡（5毫克/千克），持续5天。制备YGJ巴布剂，在第3天吗啡给药前立即贴于小鼠脱毛部位，在第5天结束时取下。第6天，腹腔注射纳洛酮（8毫克/千克）以诱发阿片类药物戒断综合征。在注射纳洛酮后立即分两个30分钟阶段进行行为观察。
就戒断严重程度评分以及跳跃、竖毛、舔前爪和转圈行为的频率而言，YGJ巴布剂显著且呈剂量依赖性地减轻了吗啡-纳洛酮诱发的实验性阿片类药物戒断反应。然而，YGJ巴布剂治疗并未改变吗啡的急性镇痛作用。
YGJ巴布剂可减轻阿片类药物依赖性及其相关的戒断症状。因此，YGJ巴布剂未来可能成为治疗阿片类药物成瘾的一种潜在疗法。
