Plasminogen activator inhibitor 2. Isolation and characterization of the promoter region of the gene.

We have isolated the promoter region and part of the coding region of the human plasminogen activator inhibitor 2 gene. The first two exons (65 and 177 base pairs, respectively) are separated by a 3.6 kilo base pair intron. More than 2 kilo base pair of the promoter region was sequenced and analyzed. The promoter region is characterized by: a) a TATAAA box located 31 base pairs upstream of the transcription initiation site; b) a 123 base pair stretch (-598 to -720) that is homologous (greater than 90% identity) to the inverted sequence of -1268 to -1380; c) a 24 base pair inverted repeat at -276 to -299 and three direct repeats; and d) several sequences that are homologous (up to 1 mismatch) to the cAMP responsive element or to binding sites for the transcription factors AP1 and AP2.