Riccio A, Lund L R, Sartorio R, Lania A, Andreasen P A, Danø K, Blasi F
International Institute of Genetics and Biophysics, CNR, Napoli, Italy.
Nucleic Acids Res. 1988 Apr 11;16(7):2805-24. doi: 10.1093/nar/16.7.2805.
The human gene for plasminogen activator inhibitor type-1 (PAI-1) has been isolated and its promoter region characterized. PAI-1 regulation by glucocorticoids, transforming growth factor-beta (TGF-beta) and the phorbol ester PMA is shown to be exerted at the promoter level. A fragment spanning 805 nucleotides of the 5' flanking and 72 of the 5' untranslated region contain information enough to promote transcription and to respond to glucocorticoids when fused to a reporter gene and transfected into human fibrosarcoma cells. A moderately repetitive DNA sequence, containing a TATA box, a GRE consensus, a Z-DNA forming sequence and two imperfect direct repeats at the extremities, is present a few nucleotides 5' of the human PAI-1 gene transcription start site, raising the possibility that this gene could have been activated by DNA insertion during evolution.
人纤溶酶原激活物抑制剂1型（PAI-1）基因已被分离出来，其启动子区域也已得到表征。研究表明，糖皮质激素、转化生长因子-β（TGF-β）和佛波酯PMA对PAI-1的调节作用是在启动子水平发挥的。一个跨越5'侧翼805个核苷酸和5'非翻译区72个核苷酸的片段，当与报告基因融合并转染到人纤维肉瘤细胞中时，包含足以促进转录并对糖皮质激素作出反应的信息。在人PAI-1基因转录起始位点上游几个核苷酸处，存在一个中度重复的DNA序列，其中包含一个TATA盒、一个糖皮质激素反应元件（GRE）共有序列、一个形成Z-DNA的序列以及两端的两个不完全直接重复序列，这增加了该基因可能在进化过程中通过DNA插入而被激活的可能性。
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