Morimoto S, Martin B M, Kishimoto Y, O'Brien J S
Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093.
Biochem Biophys Res Commun. 1988 Oct 14;156(1):403-10. doi: 10.1016/s0006-291x(88)80855-6.
Saposin D, a newly discovered heat-stable, 10 kDa glycoprotein, was isolated from Gaucher spleen and purified to homogeneity. Chemical sequencing from its amino terminus demonstrated colinearity between its amino acid sequence and the deduced amino acid sequence of the fourth domain of prosaposin, the precursor of saposin proteins. Saposin D specifically stimulates acid sphingomyelinase but has no significant effect on the other hydrolases tested.
鞘脂激活蛋白D是一种新发现的热稳定的10 kDa糖蛋白，从戈谢病脾脏中分离并纯化至同质。对其氨基末端进行化学测序表明，其氨基酸序列与鞘脂激活蛋白原（鞘脂激活蛋白的前体）第四结构域的推导氨基酸序列具有共线性。鞘脂激活蛋白D能特异性刺激酸性鞘磷脂酶，但对其他所测试的水解酶没有显著影响。
