阿维A酯与溶酶体蛋白鞘脂激活蛋白D结合会改变神经酰胺的溶解和水解。
Fenretinide binding to the lysosomal protein saposin D alters ceramide solubilization and hydrolysis.
作者信息
Milliken Brandon T, Melegari Lindy, Smith Gideon L, Grohn Kris, Wolfe Aaron J, Moody Kelsey, Bou-Abdallah Fadi, Doyle Robert P
机构信息
Department of Chemistry , Syracuse University , Syracuse , NY 13244 , USA . Email:
Department of Chemistry , State University of New York , Potsdam , NY 13676 , USA . Email:
出版信息
RSC Med Chem. 2020 Jul 14;11(9):1048-1052. doi: 10.1039/d0md00182a. eCollection 2020 Sep 1.
Abstract
Fenretinide is a synthetic retinoid pharmaceutical linked to ceramide build-up . Saposin D is an intralysosomal protein necessary for ceramide binding/degradation. We show, electronic absorption spectroscopy, fluorescence spectroscopy, and ceramide hydrolysis assays, that fenretinide is bound by saposin D { = (1.45 ± 0.49) × 10 M}, and affects ceramide solubilization/degradation.
摘要
阿维A是一种与神经酰胺积累有关的合成类视黄醇药物。鞘脂激活蛋白D是神经酰胺结合/降解所必需的溶酶体内蛋白。我们通过电子吸收光谱、荧光光谱和神经酰胺水解试验表明，阿维A与鞘脂激活蛋白D结合（解离常数Kd = (1.45 ± 0.49) × 10⁶ M），并影响神经酰胺的溶解/降解。
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RSC Med Chem. 2020 Jul 14;11(9):1048-1052. doi: 10.1039/d0md00182a. eCollection 2020 Sep 1.
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