Qiu Lin, Zhang Boyao, Liu Lang, Wang Xiaoping, Lei Chaoliang, Lin Yongjun, Zhao Jing, Ma Weihua
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
J Econ Entomol. 2017 Aug 1;110(4):1460-1464. doi: 10.1093/jee/tox126.
The mitogen-activated protein kinases (MAPKs) are conserved signal transduction pathways and broadly responsible for bacterial infection from yeast to mammals, and virus, fungi, and bacteria, specifically Bacillus thuringiensis, to insects. But little is known about the MAPK pathways in antibacterial responses in Chilo suppressalis (Walker), an important lepidopteran pest of rice. In this study, we used the bacteria of Bacillus thuringiensis, Escherichia coli, and Staphyloccocus aureus to infect C. suppressalis larvae, and the responses of MAPK pathways were analyzed. The results showed that E. coli infection induced the up-regulated expression of Csp38 and CsERK1 at 24 h postinfection (pi). Meanwhile, injection of B. thuringiensis and S. aureus resulted in strong activation of CsJNK phosphorylation at 3 h pi. These results suggest that MAPK signaling pathways play important functional roles in antibacterial responses in C. suppressalis larvae.
丝裂原活化蛋白激酶（MAPKs）是保守的信号转导途径，广泛参与从酵母到哺乳动物的细菌感染过程，以及病毒、真菌和细菌（特别是苏云金芽孢杆菌）对昆虫的感染过程。但对于水稻重要鳞翅目害虫二化螟（Walker）抗菌反应中的MAPK途径，我们所知甚少。在本研究中，我们使用苏云金芽孢杆菌、大肠杆菌和金黄色葡萄球菌感染二化螟幼虫，并分析了MAPK途径的反应。结果表明，大肠杆菌感染在感染后24小时诱导了Csp38和CsERK1的表达上调。同时，注射苏云金芽孢杆菌和金黄色葡萄球菌在感染后3小时导致CsJNK磷酸化的强烈激活。这些结果表明，MAPK信号通路在二化螟幼虫的抗菌反应中发挥重要功能作用。
