State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University , Chengdu, 610041, People's Republic of China.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , Beijing 100190, China.
Anal Chem. 2017 Jun 6;89(11):6113-6119. doi: 10.1021/acs.analchem.7b00831. Epub 2017 May 10.
This work demonstrates a highly sensitive peroxide test strip (PTS)-based enzyme-linked immunosorbent assay (ELISA) for both qualitative and quantitative detection of drugs of abuse (morphine) and disease biomarkers (interleukin-6 and HIV-1 capsid antigen p24). This color-based PTS is a commercially available product with advantages of low cost, easy operation, and portability, and it is an ideal signal readout strategy in ELISA to simplify the immunoassay procedures and enable point-of-care testing (POCT). In addition, we introduce the bioorthogonal reaction that can effectively amplify the signal by controlling the cycles of bioorthogonal reaction to achieve the desirable sensitivity depending on different analytes. The limit of detection is 0.2 ng/mL for morphine, 3.98 pg/mL for interleukin-6, and 11.6 pg/mL for detection of HIV-capsid antigen (p24). This PTS-ELISA applies to both the qualitative and quantitative detection of IL-6 and p24 in clinical serum samples with good accuracy, which provides a promising tool for the POCT in clinical diagnosis.
这项工作展示了一种基于过氧化物测试条（PTS）的高度敏感的酶联免疫吸附测定（ELISA），可用于定性和定量检测滥用药物（吗啡）和疾病生物标志物（白细胞介素-6 和 HIV-1 衣壳抗原 p24）。这种基于颜色的 PTS 是一种商业上可用的产品，具有成本低、操作简单和便携的优点，是 ELISA 中一种理想的信号读出策略，可以简化免疫分析程序并实现即时检测（POCT）。此外，我们引入了生物正交反应，可以通过控制生物正交反应的循环来有效放大信号，从而根据不同的分析物实现理想的灵敏度。吗啡的检测限为 0.2ng/mL，白细胞介素-6 的检测限为 3.98pg/mL，HIV-衣壳抗原（p24）的检测限为 11.6pg/mL。该 PTS-ELISA 可用于临床血清样本中白细胞介素-6 和 p24 的定性和定量检测，具有良好的准确性，为临床诊断中的 POCT 提供了一种有前途的工具。
