Chen Liang
College of Bioengineering, Henan University of Technology, Zhengzhou 450001, PR China; Collaborative Innovation Center of Grain Storage Security in Henan Province, Zhengzhou 450001, PR China.
J Biotechnol. 2017 Jun 10;251:124-127. doi: 10.1016/j.jbiotec.2017.04.034. Epub 2017 Apr 28.
Bacillus velezensis LM2303 is a biocontrol strain with a broad inhibitory spectrum against plant pathogens, isolated from the dung of wild yak inhabited Qinghai-Tibet plateau, China. Here we present its complete genome sequence, which consists of a single, circular chromosome of 3,989,393bp with a 46.68% G+C content. Genome analysis revealed genes encoding specialized functions for the biosynthesis of antifungal metabolites and antibacterial metabolites, the promotion of plant growth, the alleviation of oxidative stress and nutrient utilization. And the biosynthesis of antimicrobial metabolites in strain LM2303 was confirmed by biochemical analysis, while its plant growth promoting traits were confirmed by inoculation tests. Our results will establish a better foundation for further studies and biocontrol application of B. velezensis LM2303.
贝莱斯芽孢杆菌LM2303是一株对植物病原菌具有广谱抑制作用的生防菌株,从栖息于中国青藏高原的野牦牛粪便中分离得到。在此,我们展示其完整的基因组序列,该序列由一条大小为3,989,393bp的环状染色体组成,G+C含量为46.68%。基因组分析揭示了编码抗真菌代谢产物和抗菌代谢产物生物合成、促进植物生长、减轻氧化应激和营养利用等特殊功能的基因。通过生化分析证实了菌株LM2303中抗菌代谢产物的生物合成,同时通过接种试验证实了其促进植物生长的特性。我们的研究结果将为贝莱斯芽孢杆菌LM2303的进一步研究和生防应用奠定更好的基础。