爱泼斯坦-巴尔病毒DNA复制裂解性起始位点oriLyt的鉴定与特征分析

Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus.

作者信息

Hammerschmidt W, Sugden B

机构信息

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

出版信息

Cell. 1988 Nov 4;55(3):427-33. doi: 10.1016/0092-8674(88)90028-1.

DOI:10.1016/0092-8674(88)90028-1

PMID:2846181

Abstract

We have identified a cis-acting element of Epstein-Barr virus (EBV) that mediates viral DNA replication during the lytic phase of this virus's life cycle. This lytic origin of DNA replication, termed oriLyt, is complex in structure in that it contains multiple regions that are required for replication and additional DNA sequences that increase replication. One of the required regions of oriLyt can be functionally substituted by a transcriptional enhancing element. DNA replication mediated by oriLyt depends on EBV DNA polymerase and yields a concatemeric molecule. A vector, which contains both oriP (the EBV plasmid origin of replication) and oriLyt, can be maintained as a plasmid in latently EBV-infected cells and can be amplified 100- to 1000-fold in cells in which the lytic phase of the viral life cycle is induced.

摘要

我们已经鉴定出爱泼斯坦-巴尔病毒（EBV）的一个顺式作用元件，该元件在该病毒生命周期的裂解期介导病毒DNA复制。这个DNA复制的裂解起源，称为oriLyt，其结构复杂，因为它包含复制所需的多个区域以及增加复制的额外DNA序列。oriLyt的一个必需区域可以被转录增强元件进行功能替代。由oriLyt介导的DNA复制依赖于EBV DNA聚合酶，并产生一个串联分子。一种同时包含oriP（EBV质粒复制起点）和oriLyt的载体，可以作为质粒在潜伏性EBV感染的细胞中维持，并在诱导病毒生命周期裂解期的细胞中扩增100至1000倍。