Gaeini Abbasali, Baghaban Eslaminejad Mohamadreza, Choobineh Siroos, Mousavi Neda, Satarifard Sadegh, Shafieineek Leila
Department of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Int J Reprod Biomed. 2017 Feb;15(2):93-100.
Based on different studies it was shown that exercise training is an important factor in preconception and prenatal care.
The aim of this study was to determine whether regular preconception exercise training with or without exercise training during pregnancy decreases detrimental effects of maternal high fat diet on female offspring bone health.
Twenty-four C57BL/6 female mice were fed high-fat diet (35%) and were randomly divided into four groups: trained in preconception period and exercised during pregnancy (TE); trained in preconception periods but unexercised during pregnancy (TC); untrained in preconception periods but exercised during pregnancy (CE); untrained and unexercised (CC). Trained mice were subjected to a protocol of moderate endurance exercise training over a period of 4 weeks before pregnancy. TE and CE Dams groups had access to wheels throughout pregnancy until delivery. Analyses were performed on the female offspring that did not have access to running wheels or exercise training during any portion of their lives. The relative expression levels of β-catenin, Peroxisome proliferator-activated receptor ƴ (PPARƴ), osteoprotegerin (OPG), and Receptor activator of nuclear factor NF-kB ligand (RANKL) were determined by Quantitative RT-PCR (qPCR).
Exercise during pregnancy in isolation had no effect on any measure genes but exercise both before and during pregnancy affected all genes. Exercise only before pregnancy increased β-catenin and OPG and decreased PPARƴ, RANKL, and RANKL/OPG ratio (p<0.001).
This study demonstrated that maternal exercise training before and during pregnancy may modulate the risk of bone disorders in offspring of mothers fed a high-fat diet.
基于不同的研究表明，运动训练是孕前和孕期保健的一个重要因素。
本研究的目的是确定孕期进行或不进行运动训练的规律孕前运动训练是否能降低母体高脂饮食对雌性后代骨骼健康的有害影响。
24只C57BL/6雌性小鼠喂食高脂饮食（35%），并随机分为四组：孕前训练并孕期运动（TE）；孕前训练但孕期不运动（TC）；孕前未训练但孕期运动（CE）；未训练且未运动（CC）。训练的小鼠在怀孕前4周接受中等耐力运动训练方案。TE和CE组的母鼠在整个孕期直至分娩都可使用转轮。对在生命的任何阶段都无法使用转轮或进行运动训练的雌性后代进行分析。通过定量逆转录聚合酶链反应（qPCR）测定β-连环蛋白、过氧化物酶体增殖物激活受体γ（PPARγ）、骨保护素（OPG）和核因子κB受体激活剂配体（RANKL）的相对表达水平。
单独孕期运动对任何测量基因均无影响，但孕前和孕期运动均影响所有基因。仅孕前运动增加了β-连环蛋白和OPG，降低了PPARγ、RANKL以及RANKL/OPG比值（p<0.001）。
本研究表明，孕期前后母体运动训练可能调节高脂饮食母亲后代患骨骼疾病的风险。
