Su Li, Shi Lei, Liu Jian, Huang Lifei, Huang Yi, Nie Xiaomeng
School of Pharmacy, Second Military Medical University, Shanghai, China.
Mol Biosyst. 2017 May 30;13(6):1172-1181. doi: 10.1039/c7mb00025a.
Asthma is a chronic inflammatory lung disease that leads to 250 000 deaths annually. There is a need to better understand asthma by identifying new pathogenic molecules. We conducted a liquid-chromatography time-of-flight mass spectrometry (LC-Q-TOF-MS)-based metabolomics study to test for asthma and investigate the interventional mechanisms of surfactant protein A (SPA) in OVA-induced asthma mice. The results revealed that asthma disturbed 32 metabolites in 9 metabolic pathways. After SPA treatment, the metabolomics profile found in asthma was significantly reversed, shifting much closer to that of the control group, indicating that SPA has therapeutic effects against asthma. Metabolomic pathway analysis by the ingenuity pathway analysis demonstrated that several pathways including fatty acid metabolism, lipid metabolism, and purine metabolism were significantly altered in asthma. This study offers new methodologies for the understanding of asthma and the mechanisms of SPA in treating asthma.
哮喘是一种慢性炎症性肺部疾病，每年导致25万人死亡。需要通过识别新的致病分子来更好地了解哮喘。我们进行了一项基于液相色谱飞行时间质谱（LC-Q-TOF-MS）的代谢组学研究，以检测哮喘并研究表面活性蛋白A（SPA）在卵清蛋白诱导的哮喘小鼠中的干预机制。结果显示，哮喘扰乱了9条代谢途径中的32种代谢物。经SPA治疗后，哮喘患者的代谢组学特征得到显著逆转，更接近对照组，表明SPA对哮喘具有治疗作用。通过 Ingenuity 通路分析进行的代谢组学通路分析表明，包括脂肪酸代谢、脂质代谢和嘌呤代谢在内的几种通路在哮喘中发生了显著改变。本研究为理解哮喘以及SPA治疗哮喘的机制提供了新方法。
