Scarpa S, Morstyn G, Carney D N, Modesti A, Triche T J
Experimental Medicine Department, University of Rome, Italy.
Eur Respir J. 1988 Jul;1(7):639-44.
Variant subclasses of cell lines derived from small cell lung cancers have previously been characterized, having distinctive biochemical, morphological and growth properties compared to the classic lines. Both types of small cell lung cancer express features suggesting that they are derived from neuroectodermal cells. We compared the capacity of these two types of lung cancer cell lines to synthesize the extracellular matrix glycoproteins, fibronectin and laminin, and also analysed a few other non-small cell lung cancer lines, as controls. We found that the cell lines of the pure type did not produce laminin or fibronectin, whereas the cell lines of the variant type synthesized laminin, and the non-small cell lung cancer lines produced either laminin or fibronectin. These findings suggest that the variant form of small cell lung cancer may be derived from a primitive neuroectodermal cell, with both neural and epithelial features, whereas the classic type is derived from a more mature cell with predominantly neuronal features. The differences in extracellular matrix synthesis, and laminin in particular, may explain some of the in vitro and in vivo characteristics of the tumour.
此前已对源自小细胞肺癌的细胞系变异亚类进行了表征，与经典细胞系相比，它们具有独特的生化、形态和生长特性。两种类型的小细胞肺癌均表现出表明它们源自神经外胚层细胞的特征。我们比较了这两种类型的肺癌细胞系合成细胞外基质糖蛋白纤连蛋白和层粘连蛋白的能力，并分析了其他一些非小细胞肺癌细胞系作为对照。我们发现，纯类型的细胞系不产生层粘连蛋白或纤连蛋白，而变异类型的细胞系合成层粘连蛋白，非小细胞肺癌细胞系则产生层粘连蛋白或纤连蛋白。这些发现表明，小细胞肺癌的变异形式可能源自具有神经和上皮特征的原始神经外胚层细胞，而经典类型则源自具有主要神经元特征的更成熟细胞。细胞外基质合成的差异，尤其是层粘连蛋白的差异，可能解释了肿瘤的一些体外和体内特征。
