Christiansen N O
Department of Medicine and Infectious Diseases, Marselisborg Hospital, University of Aarhus, Denmark.
FEBS Lett. 1988 Nov 7;239(2):195-8. doi: 10.1016/0014-5793(88)80915-3.
The time course of superoxide generation and membrane association of protein kinase C was studied in human neutrophils stimulated by PMA, FMLP, ionomycin and A23187. The initiation of superoxide generation in PMA; ionomycin- and A23187-stimulated neutrophils was characterized by a lag period of at least 30 s in contrast to a lag period of 10-15 s in FMLP-stimulated cells. The time course of membrane association of protein kinase C in PMA-stimulated neutrophils was highly dependent upon the PMA concentration used for stimulation. However, membrane association of protein kinase C preceded superoxide generation in cells stimulated by 10-300 ng/ml PMA. FMLP, ionomycin and A23187 induced membrane association of protein kinase C in a few seconds and always before superoxide generation. It is concluded that membrane association of protein kinase C in PMA-, FMLP-, ionomycin- and A23187-stimulated neutrophils precedes superoxide generation, and thereby may be part of the mechanism initiating NADPH-oxidase activity. A simple correlation between the two parameters could not be proven, indicating that also other activation mechanisms are decisive in the activation of NADPH-oxidase.
研究了佛波酯（PMA）、N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、离子霉素和A23187刺激的人中性粒细胞中超氧化物生成的时间进程以及蛋白激酶C的膜结合情况。与FMLP刺激的细胞中10 - 15秒的延迟期相比，PMA、离子霉素和A23187刺激的中性粒细胞中超氧化物生成的起始具有至少30秒的延迟期。PMA刺激的中性粒细胞中蛋白激酶C的膜结合时间进程高度依赖于用于刺激的PMA浓度。然而，在10 - 300 ng/ml PMA刺激的细胞中，蛋白激酶C的膜结合先于超氧化物生成。FMLP、离子霉素和A23187在几秒钟内诱导蛋白激酶C的膜结合，并且总是先于超氧化物生成。结论是，在PMA、FMLP、离子霉素和A23187刺激的中性粒细胞中，蛋白激酶C的膜结合先于超氧化物生成，因此可能是启动NADPH氧化酶活性机制的一部分。这两个参数之间未能证明存在简单的相关性，表明其他激活机制在NADPH氧化酶的激活中也起决定性作用。
