Ngoupaye Gwladys Temkou, Pahaye David Bougolla, Ngondi Judith, Moto Fleur Clarisse Okomolo, Bum Elisabeth Ngo
School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa; Department of Animal Biology, University of Dschang, Dschang 67, Cameroon.
Department of Biological Science, University of Ngaoundéré, Ngaoundéré 454, Cameroon.
Biomed Pharmacother. 2017 Jul;91:350-357. doi: 10.1016/j.biopha.2017.04.061. Epub 2017 May 2.
Learning and memory are the most important executive functions performed by the human brain, the loss of which is a prominent feature in dementia. Gladiolus dalenii is traditionally used to treat a number of illnesses such as epilepsy and schizophrenia in Cameroon. This study aims to investigate the anti-amnesia effect of Gladiolus dalenii in scopolamine-induced amnesia in rats and its possible antioxidant properties in this model. Morris water maze, novel object location and recognition tasks were used to assess spatial and working memory. Male rats were treated for 12 days with saline, G. dalenii or Tacrine. Experimental animals were co-treated with scopolamine once daily from day 9 to 12. Acetylcholinesterase activity was measured in the prefrontal cortex and hippocampus. Malondialdehyde and glutathione levels were measured in the hippocampus. G. dalenii reversed memory impairment induced by scopolamine in the Morris water maze, novel object location and recognition tasks. It decreased acetylcholinesterase activity in the hippocampus and prefrontal cortex. It also decreased the level of malondialdehyde and increased the level of glutathione in the hippocampus. The results of this study show that G. dalenii ameliorates the cognitive impairment induced by scopolamine, through inhibition of oxidative stress and enhancement of cholinergic neurotransmission. It can therefore be useful for treatment of conditions associated with memory dysfunction as seen in dementia.
学习和记忆是人类大脑执行的最重要功能，而这些功能的丧失是痴呆症的一个显著特征。在喀麦隆，唐菖蒲传统上用于治疗多种疾病，如癫痫和精神分裂症。本研究旨在探讨唐菖蒲对东莨菪碱诱导的大鼠失忆的抗失忆作用及其在该模型中可能的抗氧化特性。采用莫里斯水迷宫、新物体定位和识别任务来评估空间和工作记忆。雄性大鼠用生理盐水、唐菖蒲或他克林处理12天。从第9天到第12天，实验动物每天一次联合使用东莨菪碱。测定前额叶皮质和海马中的乙酰胆碱酯酶活性。测定海马中的丙二醛和谷胱甘肽水平。唐菖蒲在莫里斯水迷宫、新物体定位和识别任务中逆转了东莨菪碱诱导的记忆障碍。它降低了海马和前额叶皮质中的乙酰胆碱酯酶活性。它还降低了海马中丙二醛的水平并提高了谷胱甘肽的水平。本研究结果表明，唐菖蒲通过抑制氧化应激和增强胆碱能神经传递来改善东莨菪碱诱导的认知障碍。因此，它可用于治疗痴呆症中所见的与记忆功能障碍相关的病症。
