Achour Amani, Derouiche Abdelkader, Barhoumi Badreddine, Kort Badreddine, Cherif Driss, Bouabdallah Sondes, Sakly Mohsen, Rhouma Khémais Ben, Touil Soufiane, Driss Mohamed Ridha, Tebourbi Olfa
Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage Tunisia, Jarzouna, 7021 Bizerte, Tunisia.
Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Tunisia.
Environ Res. 2017 Jul;156:635-643. doi: 10.1016/j.envres.2017.04.021. Epub 2017 May 12.
The aims of the present study were to investigate the current exposure levels of persistent organochlorine compounds (OCs) in adipose tissues intraoperatively collected from 40 patients over 20 years undergoing non-cancer-related surgery residing in Northern region of Tunisia (Bizerte), which constitutes an exemplary case, and examined association between levels of contamination and both socio-demographic characteristics and dietary habits. Concentration of hexachlorobenzene (HCB), hexachlorocyclohexane isomers (α-HCH, β-HCH, γ-HCH and δ-HCH), dichlorodiphenyltrichloroethane isomers (p,p'-DDT and o,p'-DDT) and metabolites (p,p'-DDE, o,p'-DDE, p,p'-DDD and o,p'-DDD) and 12 polychlorinated biphenyls (PCBs) congeners were measured using capillary gas chromatography with electron capture detector. Overall, residue levels of OCs followed the decreasing order of DDTs > PCBs > HCB > HCHs. DDTs levels ranged from 74.49 to 1834.76ngg lipid and contributing to more than 90% to the sum of organochlorine pesticides (OCPs). p,p'-DDE was the most abundant in all samples and the p,p'-DDT/p,p'-DDE ratio (range between 1.85% and 58.45%) suggesting recent and ongoing exposure to banned commercial DDT products. PCB concentrations varied from 29.27 to 322.58ngg lipid and PCB-180, PCB-153 and PCB-138 were the dominant congeners accounting for 70% of total PCBs. We did not find significant correlations between OC exposure levels and sex, parity, habitat areas and smoking habits. In females, the adipose tissue concentrations of DDTs, HCB and PCB-118 were positively correlated with age. There was statistically significant relationship between body mass index (BMI) changes and the adipose tissue levels of HCB and HCHs. No association was found between OCPs levels and dietary factors. However, our study suggests that fish consumption may be an important contributor of PCBs adipose tissue content of PCBs in Tunisian people. The presented work is highly significant, being the first study pointing out the chronic exposure to OCs in Bizerte.
本研究的目的是调查从突尼斯北部地区（比塞大）20多年来接受非癌症相关手术的40名患者术中采集的脂肪组织中持久性有机氯化合物（OCs）的当前暴露水平，该地区是一个典型案例，并研究污染水平与社会人口特征和饮食习惯之间的关联。使用带有电子捕获检测器的毛细管气相色谱法测量六氯苯（HCB）、六氯环己烷异构体（α-六氯环己烷、β-六氯环己烷、γ-六氯环己烷和δ-六氯环己烷）、二氯二苯三氯乙烷异构体（p,p'-滴滴涕和o,p'-滴滴涕）及其代谢物（p,p'-二氯二苯二氯乙烯、o,p'-二氯二苯二氯乙烯、p,p'-二氯二苯二氯乙烷和o,p'-二氯二苯二氯乙烷）以及12种多氯联苯（PCBs）同系物的浓度。总体而言，OCs的残留水平遵循滴滴涕类>多氯联苯>六氯苯>六氯环己烷类的降序排列。滴滴涕类水平在74.49至1834.76ng/g脂质之间，占有机氯农药（OCPs）总量的90%以上。p,p'-二氯二苯二氯乙烯在所有样本中含量最高，p,p'-滴滴涕/p,p'-二氯二苯二氯乙烯的比例（范围在1.85%至58.45%之间）表明近期和持续接触已被禁止的商用滴滴涕产品。多氯联苯浓度在29.27至322.58ng/g脂质之间，多氯联苯-180、多氯联苯-153和多氯联苯-138是主要同系物，占多氯联苯总量的70%。我们未发现OCs暴露水平与性别、生育状况、居住地区和吸烟习惯之间存在显著相关性。在女性中，滴滴涕类、六氯苯和多氯联苯-118的脂肪组织浓度与年龄呈正相关。体重指数（BMI）变化与六氯苯和六氯环己烷类的脂肪组织水平之间存在统计学上的显著关系。未发现OCPs水平与饮食因素之间存在关联。然而，我们的研究表明，食用鱼类可能是突尼斯人脂肪组织中多氯联苯含量的一个重要贡献因素。本研究具有高度重要性，是第一项指出比塞大存在OCs慢性暴露的研究。
